{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:24:34Z","timestamp":1787505874673,"version":"build-2736575974"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biofilm formation and virulence factor production by\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    are identified as the main mechanisms of its antibiotic resistance and pathogenicity. In this context, the study of the chemical composition of three Algerian essential oils (EOs) and the screening of their antibacterial, antibiofilm, and virulence factor inhibitory activities enabled us to select the thyme EO as the best oil to control the\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strain isolated from hospital environments. This EO, composed essentially of thymol (55.82%) associated with carvacrol, had an anti\u2010adhesive activity of 69.8% at a concentration of 5\u00a0\u00b5L\/mL and a biofilm eradication activity of 74.86% at a concentration of 2.5\u00a0\u00b5L\/mL. In addition, this EO was able to inhibit\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    twitching motility by 100% at a concentration of 2.5\u00a0\u00b5L\/mL. Pyocyanin was inhibited by 99.33% at a thyme EO concentration of 1.25\u00a0\u00b5L\/mL. Rhamnolipids were significantly inhibited by 63.33% in the presence of thyme EO at a concentration of 1.25\u00a0\u00b5L\/mL after 24\u00a0h of incubation. Molecular docking showed that carvacrol and thymol can bind to the three quorum sensing receptors in\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , RhlR, LasR, and PqsR, with good affinities, which can inhibit or modulate biofilm formation and the production of certain virulence factors.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402691","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T22:47:23Z","timestamp":1736290043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of Antibacterial Activity in Some Algerian Essential Oils and Selection of\n                    <i>Thymus vulgaris<\/i>\n                    as a Potential Biofilm and Quorum Sensing Inhibitor Against\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Fatima Zahra","family":"Mohammed Aggad","sequence":"first","affiliation":[{"name":"Laboratory of Applied Hydrology and Environment Faculty of Science and Technology University of Ain Temouchent  Ain Temouchent Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-0737","authenticated-orcid":false,"given":"Faiza","family":"Ilias","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Hydrology and Environment Faculty of Science and Technology University of Ain Temouchent  Ain Temouchent Algeria"},{"name":"Laboratory of Applied Genetic in Agriculture, Ecology and Public Health University of Tlemcen  Tlemcen Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fares","family":"Elghali","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Screening Processes at Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Mrabet","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Screening Processes at Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5364-377X","authenticated-orcid":false,"given":"Imad Abdelhamid","family":"El Haci","sequence":"additional","affiliation":[{"name":"Centre de Recherche Scientifique et Technique en Analyses Physico\u2010Chimiques CRAPC  Bou\u2010Ismail Tipaza Algeria"},{"name":"Laboratoire des Produits Naturels, Facult\u00e9 SNV\u2010STU Universit\u00e9 de Tlemcen  Tlemcen Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Aifa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Screening Processes at Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Mnif","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Screening Processes at Centre of Biotechnology of Sfax  Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2015.11.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.09.106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2017.03.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.36290\/csf.2015.058"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2013.06.013"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.94"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.89"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgeb.2018.07.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11051345"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2005.00012.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","unstructured":"R. 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