{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T13:35:31Z","timestamp":1781962531299,"version":"3.54.5"},"reference-count":63,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T00:00:00Z","timestamp":1740528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Zygophyllaceae R.Br. is a family with a high level of variability in plasticity that results in many conflicts about its taxa ranking and authenticating processes. Five selected Egyptian species belonging to genera, <jats:italic>Fagonia<\/jats:italic>, <jats:italic>Tribulus<\/jats:italic>, and <jats:italic>Zygophyllum<\/jats:italic> were applied for a comparative chemical and molecular study to assess their range of variations. The plants were evaluated for their 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) radical scavenging activity. Liquid chromatography\u2010electrospray ionization tandem mass spectrometry profiling of the aqueous methanol extracts of the investigated plants and Global Natural Product Social molecular networking tentatively identified 174 compounds belonging to various chemical classes. Flavonoids are predominant, and myricetin derivatives were reported for the first time from <jats:italic>Fagonia glutinosa<\/jats:italic>. Diethyl ether extract analysis with gas chromatography with flame ionization detection detected 10 unsaturated and 13 saturated fatty acids. <jats:italic>T. terrestris<\/jats:italic> possessed the largest concentration of unsaturated fatty acids (up to 76.39%) whereas <jats:italic>Z. simplex<\/jats:italic> had the highest concentration of the saturated ones (76.82%). DNA fingerprinting was performed using Inter\u2010Simple Sequence Repeat markers and variations with 75.32% polymorphism were detected. Chemical and molecular data suggest the distinction of <jats:italic>Tribulus<\/jats:italic> sp. from the other studied taxa and grouping <jats:italic>Fagonia<\/jats:italic> close to <jats:italic>Zygophyllum<\/jats:italic>. Moreover, the DPPH assay showed weak to moderate radical scavenging activity at 500\u00a0\u00b5g\/mL with cell viability ranging from 63.5% to 90.1%.<\/jats:p>","DOI":"10.1002\/cbdv.202402695","type":"journal-article","created":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T08:59:41Z","timestamp":1739609981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolite\u2010Profiling and DNA\u2010Fingerprinting of Some Selected Species of Family Zygophyllaceae With Assessment of Their Bioactive Potential"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1979-511X","authenticated-orcid":false,"given":"Amal A.","family":"Draz","sequence":"first","affiliation":[{"name":"Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute National Research Centre  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-2781","authenticated-orcid":false,"given":"Mai M.","family":"Farid","sequence":"additional","affiliation":[{"name":"Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute National Research Centre  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7609-9383","authenticated-orcid":false,"given":"Hasnaa A.","family":"Hosni","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department Faculty of Science Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0068-0224","authenticated-orcid":false,"given":"Eman M.","family":"Shamso","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department Faculty of Science Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3914-3423","authenticated-orcid":false,"given":"Salwa A.","family":"Kawashty","sequence":"additional","affiliation":[{"name":"Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute National Research Centre  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3657-7410","authenticated-orcid":false,"given":"Sameh R.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute National Research Centre  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/2666648"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-32219-1_56"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.12705\/675.11"},{"key":"e_1_2_8_5_1","volume-title":"Flora of Egypt: Volume Two (Geraniaceae\u2014Boraginaceae)","author":"Boulos L.","year":"2000"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00606-003-0007-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2008.02.019"},{"key":"e_1_2_8_8_1","unstructured":"D.Van Huyssteen Morphologisch\u2010Systematische Studien \u00fcber die Gattung Zygophyllum. 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