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Gas chromatography\u2010mass spectrometry was used for the volatile component analysis of <jats:italic>C. hyalolepis<\/jats:italic>, while liquid chromatography\u2010mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2\u2010diphenyl\u20101\u2010picrylhydrazyl, 2,2'\u2010azino\u2010bis\u20103\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid, cupric ion\u2010reducing antioxidant capacity, and <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010dimethyl\u2010p\u2010phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that <jats:italic>C. hyalolepis<\/jats:italic> has antioxidant properties and is a highly efficient antibacterial agent against <jats:italic>Bacillus megaterium<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, <jats:italic>Bacillus subtilis<\/jats:italic> subsp. <jats:italic>spizizenii<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>Klebsiella aerogenes<\/jats:italic>, and <jats:italic>Candida albicans<\/jats:italic>. Additionally, <jats:italic>C. hyalolepis<\/jats:italic> contains many volatile compounds, such as 2\u2010methyl propanoic acid, 3,5\u2010octadien\u20102\u2010one, 2,6\u2010dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta\u2010cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7\u2010glucuronide, quinic acid, and 4\u2010hydroxybenzoic acid. Moreover, <jats:italic>C. hyalolepis<\/jats:italic> was shown to inhibit xanthine oxidase and elastase.<\/jats:p>","DOI":"10.1002\/cbdv.202402768","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T13:34:13Z","timestamp":1741181653000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phytochemical Profile, Antimicrobial, Antioxidant, Anti\u2010xanthine Oxidase, and Anti\u2010elastase Activities of <i>Centaurea hyalolepis<\/i>: An In Silico and In Vitro Analysis"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6570-8128","authenticated-orcid":false,"given":"Leyla","family":"Ercan","sequence":"first","affiliation":[{"name":"Department of Health, Culture, and Sports Mardin Artuklu University  Mardin T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8129-4394","authenticated-orcid":false,"given":"Cemile G\u00fcnbegi","family":"\u00c7ali\u015fkan","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques Vocational Higher School of Health Services, Mardin Artuklu University  Mardin T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3033-4349","authenticated-orcid":false,"given":"Hasan","family":"Akan","sequence":"additional","affiliation":[{"name":"Department of Biology Harran University Faculty of Arts and Sciences  Sanliurfa T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2018.07.016"},{"key":"e_1_2_9_3_1","first-page":"343","article-title":"A Revision of Centaurea (Compositae\u2010Cardueae) in the Flora of Iraq","volume":"7","author":"Wagenitz G.","year":"2006","journal-title":"Rostaniha"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.15553\/c2020v751a13"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.47495\/okufbed.1289996"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(00)00399-8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14409"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202201049"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/S40415\u2010023\u201000949\u2010X"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28041845"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advnut.2022.10.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc3010011"},{"key":"e_1_2_9_13_1","first-page":"298","article-title":"Medicinal Plant Crops\u2014Important Source of High Value\u2010added Products","volume":"59","author":"Grigore A.","year":"2016","journal-title":"Scientific Papers\u2013Series A. 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