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Polylactic\u2010<jats:italic>co<\/jats:italic>\u2010glycolic acid (PLGA) copolymer is recognized as an effective drug delivery carrier, exhibiting excellent biochemical properties. In this experiment, VPP was encapsulated with PLGA to form VPP PLGA nanoparticles (VPP\u2013PLGA NPs). The morphological structure and immunomodulatory effects of VPP\u2013PLGA NPs were evaluated. The particle size of VPP\u2013PLGA NPs was reduced compared to VPP, and the optimal preparation conditions were as follows: The ratio of the organic phase to the internal aqueous phase was 8:1, the ratio of the external aqueous phase to the primary emulsion was 7:1, and the concentration of PLGA was 20\u00a0mg\/mL. Additionally, VPP\u2013PLGA NPs significantly increased the nitric oxide (NO) content, IL\u20104, and IFN\u2010\u03b3 levels in RAW264.7 cells, as well as enhanced their phagocytic activity. Furthermore, VPP\u2013PLGA NPs were found to increase NO content and IFN\u2010\u03b3 secretion in bone marrow\u2013derived dendritic cells (BMDCs). These findings suggest that VPP\u2013PLGA NPs could enhance the immune activity and may be utilized as a VPP delivery system for inducing strong immune responses.<\/jats:p>","DOI":"10.1002\/cbdv.202402819","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T12:28:22Z","timestamp":1732710502000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preparation, Characterization, and Immune Activity of <i>Viola philippica<\/i> Polysaccharide PLGA Nanoparticles"],"prefix":"10.1002","volume":"22","author":[{"given":"Tiantian","family":"Wei","sequence":"first","affiliation":[{"name":"Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, College of Animal Sciences Fujian Agriculture and Forestry University  Fuzhou P. R. 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