{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T17:33:05Z","timestamp":1769103185989,"version":"3.49.0"},"reference-count":70,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The investigation of natural products is a valid strategy to identify compounds active against coronaviruses. We herein report the screening of <jats:italic>Baccharis<\/jats:italic> and <jats:italic>Campovassouria<\/jats:italic> plant species against the murine betacoronavirus murine hepatitis virus\u20103 (MHV\u20103), assays that can be carried out in BSL\u20102 facilities. These genera occur exclusively in the Americas and are a source of secondary metabolites with antiviral and anti\u2010inflammatory activity. <jats:italic>Campovassouria cruciata<\/jats:italic> emerged as the most active species from the screening, and its phytochemical investigation afforded the discovery of a new isomer of leptocarpin, 3\u2010<jats:italic>epi<\/jats:italic>\u2010leptocapin (<jats:bold>1<\/jats:bold>), in addition to the sesquiterpenes lactones leptocarpin (<jats:bold>2<\/jats:bold>) and arturin (<jats:bold>3<\/jats:bold>), along with umbelliferone (<jats:bold>4<\/jats:bold>). The structures were elucidated by extensive mono\u2010 and two\u2010dimensional <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR data analysis. The antiviral activity of the isolated compounds was also assayed in Calu\u20103 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102). All compounds inhibited SARS\u2010CoV\u20102 replication, with a reduction in viral load of approximately 1.7\u20132.0\u00a0log when tested at 7.5\u00a0\u00b5M. To evaluate potential anti\u2010inflammatory activity, compounds were tested in A549 cells stimulated with IFN\u2010\u03b3 and TNF\u2010\u03b1. Both 3\u2010<jats:italic>epi<\/jats:italic>\u2010leptocarpin (<jats:bold>1<\/jats:bold>) and umbelliferone (<jats:bold>4<\/jats:bold>) inhibited interleukin\u20106 (IL\u20106) and IL\u20108 release significantly. Compound <jats:bold>1<\/jats:bold> reduced the production of reactive oxygen species (ROS) in A549 cells, as assessed by flow cytometry using DCFH\u2010DA, whereas <jats:bold>4<\/jats:bold> exhibited ROS values seven\u2010fold higher than the basal level. Our results highlight <jats:italic>C. cruciata<\/jats:italic> as a source of promising bioactive compounds, which deserve future investigations to explore their potential for the development of new therapeutic agents against inflammatory and viral diseases.<\/jats:p>","DOI":"10.1002\/cbdv.202402824","type":"journal-article","created":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T21:26:32Z","timestamp":1744493192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Activity of Sesquiterpene Lactones and Umbelliferone From <i>Campovassouria cruciata<\/i> on SARS\u2010CoV\u20102 Replication and on the Release of Pro\u2010Inflammatory Cytokines in Lung Cells"],"prefix":"10.1002","author":[{"given":"Maria Beatriz de O.","family":"Rabelo","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00e1tia M.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo C.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra C. M.","family":"Frade","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 D. de S.","family":"Filho","sequence":"additional","affiliation":[{"name":"CiPharma\u2014Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas School of Pharmacy, Universidade Federal de Ouro Preto  Ouro Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Heiden","sequence":"additional","affiliation":[{"name":"Herb\u00e1rio ECT\u2014Embrapa Clima Temperado  Pelotas Rio Grande do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldo W.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ecology and Evolution, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabella Tha\u00eds","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Virology, Department of Microbiology, Immunology, and Parasitology Universidade Federal de Santa Catarina  Florian\u00f3polis Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fern\u00e3o C.","family":"Braga","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9939-6900","authenticated-orcid":false,"given":"Rodrigo M.","family":"P\u00e1dua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030781"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030747"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70690-X"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00483-20"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_9_8_1","unstructured":"World Health Organization COVID\u201019 Weekly Epidemiological Update(WHO 2024)."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000004845"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43440-020-00155-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.06.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31210-w"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01285"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.561334"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-1742095"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.9790\/5736-07810108"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765201820170653"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-40422005000100017"},{"key":"e_1_2_9_19_1","volume-title":"Tratamento Taxon\u00f4mico e Sinopse De Symphyopappus","author":"Hattori E. 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