{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:46:15Z","timestamp":1773013575336,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Snakebite envenomation is a public health issue that can lead to mortality and physical consequences. It is estimated that 5.4 million venomous snake bites occur annually, with 130\u00a0000 deaths and 400\u00a0000 amputations. The available treatment is serotherapy, which is inefficient in preventing physical sequelae. Thus, it is critical to seek efficient treatments. Here, we assessed the efficacy of fruits from\n                    <jats:italic>Clusia fluminensis<\/jats:italic>\n                    harvested at different time periods over 1 year against the toxicity of\n                    <jats:italic>Bothrops jararacussu<\/jats:italic>\n                    venom. The extracts were incubated with\n                    <jats:italic>B. jararacussu<\/jats:italic>\n                    venom, and the in vitro (coagulant and proteolytic) and in vivo (hemorrhagic, edematogenic, and lethal) activities of the venom were assessed. Moreover,\n                    <jats:italic>B. jararacussu<\/jats:italic>\n                    venom was injected 30\u00a0min before the extract injection to evaluate hemorrhagic activity. Little variation in the chemical composition of the fruit extracts from\n                    <jats:italic>C. fluminensis<\/jats:italic>\n                    or their ability to inhibit the toxic effects of\n                    <jats:italic>B. jararacussu<\/jats:italic>\n                    venom was observed. When administered after venom injection, the\n                    <jats:italic>C. fluminensis<\/jats:italic>\n                    extracts inhibited hemorrhage caused by\n                    <jats:italic>B. jararacussu<\/jats:italic>\n                    venom. Thus, the plant collection period should be considered to achieve the highest antivenom potential.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402941","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T15:39:50Z","timestamp":1735918790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of the Effects of Harvesting Period of\n                    <i>Clusia fluminensis<\/i>\n                    Planch. and Triana Fruits on Neutralization of the Toxic Activities of\n                    <i>Bothrops jararacussu<\/i>\n                    Snake Venom"],"prefix":"10.1002","volume":"22","author":[{"given":"Aldo","family":"Rodrigues da Silva","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia","family":"Pietroluongo","sequence":"additional","affiliation":[{"name":"Experimental Pharmacotechnics Laboratory Oswaldo Cruz Foundation  Rio de Janeiro Brazil"},{"name":"Postgraduate Program in Science Applied to Health Products Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Cl\u00e1udia","family":"Rodrigues da Silva","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eladio Flores","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry of Proteins From Animal Venoms Research and Development Division Ezequiel Dias Foundation  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno S\u00e9rgio","family":"do Amaral","sequence":"additional","affiliation":[{"name":"Federal Institute of Education, Science, and Technology of S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina","family":"Anholeti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Quintella","family":"Lob\u00e3o","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Leda","family":"Valverde","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Science Applied to Health Products Federal Fluminense University  Rio de Janeiro Brazil"},{"name":"Department of Organic Chemistry Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selma Ribeiro","family":"de Paiva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Science Applied to Health Products Federal Fluminense University  Rio de Janeiro Brazil"},{"name":"Department of General Biology Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5265-5258","authenticated-orcid":false,"given":"Andr\u00e9 Lopes","family":"Fuly","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"1","volume-title":"Control of Neglected Tropical Diseases","author":"World Health Organization","year":"2019"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005662"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61159-4"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080090"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000569"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins15110648"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6315-0"},{"key":"e_1_2_11_9_1","first-page":"791","article-title":"Variability of Bothrojaracin Isoforms and Other Venom Principles in Individual Jararaca (Bothrops jararaca) Snakes Maintained Under Seasonally Invariant Conditions","volume":"120","author":"Monteiro R. 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