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Phytochemical analyses, antioxidant, antibacterial, anti\u2010inflammatory, antidiabetic and anticancer assays were performed. The effect of <jats:italic>K. calycina<\/jats:italic> leaf extract on the germination of radish and amaranth seeds was determined. The HRLC\u2013MS analysis revealed that the ethanol, methanol and ethyl acetate extracts showed the presence of 46, 64 and 44 compounds, respectively. The ethanolic extract of <jats:italic>K. calycina<\/jats:italic> leaf exhibited the highest DPPH scavenging (IC<jats:sub>50<\/jats:sub> value\u00a0=\u00a028.37\u00a0\u00b1\u00a00.03\u00a0\u00b5g\/mL), phosphomolybdenum reduction (IC<jats:sub>50<\/jats:sub> value\u00a0=\u00a062.11\u00a0\u00b1\u00a00.14\u00a0\u00b5g\/mL), anti\u2010inflammatory activity (IC<jats:sub>50<\/jats:sub> value\u00a0=\u00a060.38\u00a0\u00b1\u00a00.47\u00a0\u00b5g\/mL), \u03b1\u2010amylase inhibition (IC<jats:sub>50<\/jats:sub> value\u00a0=\u00a063.94\u00a0\u00b1\u00a00.55\u00a0\u00b5g\/mL), \u03b1\u2010glucosidase inhibition (IC<jats:sub>50<\/jats:sub> value\u00a0=\u00a025.54\u00a0\u00b1\u00a00.38\u00a0\u00b5g\/mL) and antimicrobial activity against <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> in comparison to the other extracts. The ethyl acetate extract showed cytotoxicity towards A549 cells with an IC<jats:sub>50<\/jats:sub> value of 22.06\u00a0\u00b1\u00a00.9\u00a0\u00b5g\/mL. The germination percentage for radish and amaranth seeds were 63.33%\u00a0\u00b1\u00a00.2% and 76.66%\u00a0\u00b1\u00a00.1%, respectively. The in silico studies showed the binding affinity of the phytocompounds towards the protein targets for antibacterial, antidiabetic and anticancer activity.<\/jats:p>","DOI":"10.1002\/cbdv.202403132","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T16:57:07Z","timestamp":1741712227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Phytochemical Profiling, Bioactive Potential and In Silico Analysis of <i>Kydia calycina<\/i> Roxb. Leaf Extracts"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4851-7992","authenticated-orcid":false,"given":"Nayan Kumar","family":"Sishu","sequence":"first","affiliation":[{"name":"Department of Biotechnology School of Biosciences and Technology VIT Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5292-0122","authenticated-orcid":false,"given":"Chinnadurai Immanuel","family":"Selvaraj","sequence":"additional","affiliation":[{"name":"Department of Genetics and Plant Breeding VIT School of Agricultural Innovations and Advanced Learning (VAIAL) VIT Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.5267\/j.ccl.2023.1.005"},{"key":"e_1_2_12_3_1","first-page":"162","article-title":"A Comprehensive Review on Pharmacological Properties of Kydia Calycina","volume":"7","author":"Goyal P. 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