{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:09:37Z","timestamp":1784898577259,"version":"3.55.0"},"reference-count":61,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ethnic Mising women of northeast India uses the dried root of <jats:italic>Paederia foetida<\/jats:italic> Linn. for fertility regulation. However, there is no scientific literature regarding female reproductive concerns so far. Therefore, this study was undertaken to know the phytocompounds present in the root of <jats:italic>P. foetida<\/jats:italic> and investigate the effect of <jats:italic>P. foetida<\/jats:italic> on the estrous cycle, ovarian, and uterine histoarchitecture. Methanolic root extract (MRE) was prepared at the doses 250 and 500\u00a0mg\/kg bw (compared to 17\u03b2\u2010estradiol as a reference drug) and was tested in ovary\u2010intact mice for 21\u00a0days. MRE at 500\u00a0mg\/kg bw\u2010treated mice resulted in degenerative ovarian histoarchitecture with a significant increase (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) in atretic follicles and a significant decrease (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) in a number of endometrial gland and disruptive reproductive hormones. Compounds like stigmasterol, having profound antifertility agents, were found in the GC\u2013MS analysis. As per molecular docking, the phytocompounds that showed the highest binding affinity were \u03b3\u2010sitosterol (\u221211.25\u00a0kcal\/mol) and ergosterol (\u221212.92\u00a0kcal\/mol) against human estrogen receptor alpha and beta (hER\u03b1 and hER\u03b2), respectively. This study is the first\u2010ever report of <jats:italic>P. foetida<\/jats:italic> roots extract on reproductive parameters and compounds of <jats:italic>P. foetida<\/jats:italic> showing binding affinity with hER. This study provides scientific evidence that supports the traditional usage of <jats:italic>P. foetida<\/jats:italic> Linn. for fertility regulation, which will further help in the standardization and development of drugs for fertility control.<\/jats:p>","DOI":"10.1002\/cbdv.202403203","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:32:05Z","timestamp":1741685525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Vivo Investigation of Root Extract of <i>Paederia foetida<\/i> Linn. on the Reproductive Functions of Female Albino Mice and In Silico Validation With Human Estrogen Receptors"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9584-5150","authenticated-orcid":false,"given":"Sivani","family":"Mili","sequence":"first","affiliation":[{"name":"Animal Physiology and Biochemistry Laboratory, Department of Zoology Gauhati University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambalika","family":"Phonglo","sequence":"additional","affiliation":[{"name":"Catalysis and Molecular Modelling Lab (CMML), Department of Chemical Sciences Tezpur University  Tezpur Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0744-9807","authenticated-orcid":false,"given":"Taslema","family":"Begum","sequence":"additional","affiliation":[{"name":"Animal Physiology and Biochemistry Laboratory, Department of Zoology Gauhati University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8866-6186","authenticated-orcid":false,"given":"Yungkham Rajeevkumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Zoology Assam Don Bosco University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9929-7971","authenticated-orcid":false,"given":"Jogen Chandra","family":"Kalita","sequence":"additional","affiliation":[{"name":"Animal Physiology and Biochemistry Laboratory, Department of Zoology Gauhati University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_14_2_1","unstructured":"United Nations \u201cRevision of World Population Prospects \u201d UN Report (United Nations March 8 2023)."},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.NAJ.0000654304.29632.a7"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2006.05.073"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.21608\/ejvs.2024.311779.2316"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23371"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.629591"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.7897\/2230\u20108407.09698"},{"issue":"2","key":"e_1_2_14_9_1","first-page":"108","article-title":"Some Ayurvedic Important Plants From District Kamrup (Assam)","volume":"3","author":"Deka L.","year":"1983","journal-title":"Ancient Science of Life"},{"key":"e_1_2_14_10_1","first-page":"166","volume-title":"Charak Samhita","author":"Shree Gulabkunverba Ayurvedic Society","year":"1949"},{"key":"e_1_2_14_11_1","volume-title":"Pharmacognosy of Indigenous Drugs","author":"Raghunath K.","year":"1982"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11676-013-0369-2"},{"key":"e_1_2_14_13_1","first-page":"410","article-title":"Wild Edible Plants of Meghalaya, North East India","volume":"6","author":"Jasmine T. 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