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IMS showed that kabiramides, the major trisoxazole macrolides in <jats:italic>P. nux<\/jats:italic>, were allocated preferentially in the external tissues of the sponge. The deposition covered the outer tissue layers from the pinadoderm through mesohyl and ectosome to the outer portion of the choanosome. The presence of each macrolide varied independently from one sponge specimen to others and showed no inductive or suppressive influences on each other compounds nor particular preferences on any specific cell types or tissues. The results provided new and additional evidence of the specificity of the sponge's toxic metabolite distribution at the tissue level. The outward distribution of the toxic macrolides in <jats:italic>P. nux<\/jats:italic> aligned with the optimal defense theory, and suggested that the sponge has evolved its chemical defensive mechanisms purposely for the best protection of the most vulnerable, newly generated branches of the colony.<\/jats:p>","DOI":"10.1002\/cbdv.202403215","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T22:19:13Z","timestamp":1740089953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tracing Trisoxazole Macrolide Deposition in the Tissues of the <i>Penares nux<\/i> Sponge With Matrix\u2010Assisted Laser Desorption Ionization Imaging Mass Spectrometry"],"prefix":"10.1002","volume":"22","author":[{"given":"Akkarapol","family":"Butsuri","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences Prince of Songkla 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