{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:41:02Z","timestamp":1787787662327,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Astragalus tokatensis<\/jats:italic>\n                    Fisch. is a local endemic species, and no studies have been conducted on this species. Hexane, dichloromethane, methanol, and water extracts were obtained from the roots, leaves, and flowers of\n                    <jats:italic>A. tokatensis<\/jats:italic>\n                    . The phytochemical analyses of hexane and methanol extracts were identified and characterized using gas chromatography\u2010mass spectrometry (GC\u2010MS) and liquid chromatography\u2010high resolution mass spectrometry (LC\u2010HRMS), respectively, for the first time. A total of 11 fatty acids and eight flavonoid derivatives were detected by GC\u2010MS and LC\u2010HRMS analyses, respectively. The total antioxidant level (TAL) and total oxidant level (TOL) of the extracts were investigated. TAL and TOL values were expressed as mM Trolox equiv\/L and \u00b5M H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    equiv\/L, respectively. \u0130t was determined that all extracts showed high antioxidant activity according to reference value &gt;2.0, and the highest antioxidant activity was found in the water extract of the leaf with 4.292\u00a0mM Trolox equiv\/L. In addition, the \u03b1\u2010amylase enzyme activity of all the extracts was examined, and it was found that the extracts had no significant effect on \u03b1\u2010amylase activity. In addition, the DNA\u2010protective activities of the leaf and flower extracts were determined by gel electrophoresis, and it was observed that all flower extracts had high DNA\u2010protective activity. Moreover, Docking assays showed that the limited interactions of sinapinic acid and salicylic acid, the most abundant in the methanol extracts, with \u03b1\u2010amylase, supported the weak inhibitory effects observed experimentally in the methanol extracts.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202403236","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T06:20:29Z","timestamp":1737440429000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Elucidating the Biological Potential and DNA Protective Activities of Astragalus tokatensis Fisch: Gas Chromatography\u2010Mass Spectrometry and Liquid Chromatography\u2010High Resolution Mass Spectrometry\u2010Based Characterization and Molecular Docking Studies"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5819-4894","authenticated-orcid":false,"given":"Bedrettin","family":"Selvi","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Arts and Sciences Tokat Gaziosmanpasa University  Tokat Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahide K\u00fcbra","family":"Zanbak","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Arts and Sciences Tokat Gaziosmanpasa University  Tokat Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"The Natural History of Medicinal Plants","author":"Sumner J.","year":"2000"},{"key":"e_1_2_11_3_1","first-page":"965","article-title":"Medicinal Plants in Therapy","volume":"63","author":"Farnsworth N. 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