{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:20:11Z","timestamp":1789068011522,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Digitalis purpurea<\/jats:italic> L. is traditionally valued for its cardiac glycosides, but its pesticidal properties remain underexplored. This study evaluated its nematicidal efficacy against <jats:italic>Meloidogyne incognita<\/jats:italic>, focusing on acetylcholinesterase (AChE) inhibition as a potential mechanism. Extracts obtained using Soxhlet extraction with hexane (DPHE), dichloromethane (DPDE), and methanol (DPME) were analyzed via GC\u2013MS. DPHE contained fatty acids, including hexadecenoic acid (20.38%) and linoleic acid (14.43%). DPDE was rich in bioactives like gitogenin (8.89%) and digitoxigenin (6.8%). DPME featured \u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucopyranose, 1,6\u2010anhydro (15.55%), and smilagenin (5.49%). Nematicidal assays showed DPDE achieved 100% mortality at 1000\u00a0\u00b5g\/mL and 91.23% egg\u2010hatching inhibition. It also demonstrated 87.88% AChE inhibition, surpassing physostigmine (81.75%). Molecular docking revealed strong interactions between DPDE compounds and AChE. These findings suggest <jats:italic>D. purpurea<\/jats:italic>, particularly its dichloromethane extract, as a promising eco\u2010friendly nematicidal agent.<\/jats:p>","DOI":"10.1002\/cbdv.202403274","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T23:45:56Z","timestamp":1742946356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical Insights and Acetylcholinesterase\u2010Targeted Nematicidal Activity of <i>Digitalis purpurea<\/i> L. Extracts in Biological Control of <i>Meloidogyne incognita<\/i>"],"prefix":"10.1002","author":[{"given":"Himani","family":"Karakoti","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Basic Sciences and Humanities G. B. 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S.","family":"Rawat","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Basic Sciences and Humanities G. B. Pant University of Agriculture and Technology  Pantnagar Uttarakhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4076-2443","authenticated-orcid":false,"given":"Mozaniel Santana","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Campus de Pesquisa\u2014Museu Paraense Em\u00edlio Goeldi\u2014Botany Coordination  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678\u20104499.20190359"},{"key":"e_1_2_9_3_1","volume-title":"Emerging Trends in Plant Pathology","author":"Khan M. 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