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Forty\u2010eight pure isolates of fungal endophytes were obtained from cultures of both young and old leaves across eleven different branch types. Furthermore, four specific fungal strains were selected for the evaluation of anticancer activity: <jats:italic>Fusarium<\/jats:italic> sp. strain PCL\u201034\u2010B, <jats:italic>Phomopsis<\/jats:italic> sp. strain PCL\u2010Y\u201048, <jats:italic>Lasiodiplodia<\/jats:italic> sp. strain PCL\u2010O\u20109, and <jats:italic>Phomopsis<\/jats:italic> sp. strain PCL\u2010O\u201023. The ethyl acetate and methanol extracts of these fungal endophytes demonstrated intriguing results, with the ethyl acetate extracts of PCL\u2010Y\u201048 exhibiting the most significant cytotoxic activity among the various fungal endophyte extracts. The half\u2010maximal inhibitory concentration values were recorded at 10.52 and 33.54\u00a0mg\/L against the A549 lung cancer and MG\u201063 osteosarcoma cells, respectively. The identification of chemical substances reveals a range of components believed to play a role in their anticancer effects.<\/jats:p>","DOI":"10.1002\/cbdv.202403347","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T22:34:47Z","timestamp":1740522887000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Biodiversity and Anticancer Potential of Fungal Endophytes from <i>Peronema canescens<\/i> Jack"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8909-1452","authenticated-orcid":false,"given":"Asih","family":"Triastuti","sequence":"first","affiliation":[{"name":"Department of Pharmacy Universitas Islam Indonesia  Yogyakarta Indonesia"},{"name":"Department of Pharmacy Endophyte Research Center, Universitas Islam Indonesia  Yogyakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Senya Putri","family":"Amalia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Sekolah Tinggi Ilmu Kesehatan ISFI  Banjarmasin, South Kalimantan Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Ilyas","sequence":"additional","affiliation":[{"name":"Research Center for Biosystematics and Evolution National Research and Innovation Agency (BRIN)  Bogor Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amila","family":"Pramisandi","sequence":"additional","affiliation":[{"name":"Research Center for Applied Microbiology National Research and Innovation Agency (BRIN)  Bogor Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andika Wahyu","family":"Wardana","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Universitas Islam Indonesia  Yogyakarta Indonesia"},{"name":"Department of Pharmacy Endophyte Research Center, Universitas Islam Indonesia  Yogyakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratu Aini","family":"Lubis","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Universitas Islam Indonesia  Yogyakarta Indonesia"},{"name":"Department of Pharmacy Endophyte Research Center, Universitas Islam Indonesia  Yogyakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatang Shabur","family":"Julianto","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universitas Islam Indonesia  Yogyakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farida Juliantina","family":"Rachmawaty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Universitas Islam Indonesia  Yogyakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Sukmarini","sequence":"additional","affiliation":[{"name":"Research Center for Applied Microbiology National Research and Innovation Agency (BRIN)  Bogor Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"World Health Organization.WHO Fact Sheet: Cancer. 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