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All the synthesized compounds were evaluated for their biological potential in a cell painting assay. Among the synthesized spiroisatin derivatives, compound <jats:bold>IV\u2010p<\/jats:bold> exhibited significant activity in inducing cellular morphological changes, with an induction value of 30.6%. In addition, some compounds showed high biosimilarities with marketed drugs. Specifically, the compounds <jats:bold>IV\u2010n<\/jats:bold> and <jats:bold>IV\u2010p<\/jats:bold> showed a high biosimilarity with the orally active iron chelator deferasirox, and <jats:bold>IV\u2010m<\/jats:bold> showed a high biosimilarity with the kinase inhibitor alisertib. Furthermore, compounds <jats:bold>IV\u2010p<\/jats:bold> showed significant inhibition against human breast cancer (MDA\u2010MB\u2010231\u00a0=\u00a082.37%) and colorectal carcinoma cell lines (HCT\u2010116\u00a0=\u00a086.25%) during preliminary investigations. Moreover, it was revealed through molecular docking analysis that <jats:bold>IV\u2010p<\/jats:bold> possesses a good binding score against ferroportin and Aurora A kinase (\u22129.3 and \u22129.2\u00a0kcal\/mol), which are quite comparable with the deferasirox (\u22129.2\u00a0kcal\/mol) and alisertib (\u22129.8\u00a0kcal\/mol). Pharmacokinetic studies revealed that the synthesized conjugates have good oral bioavailability, balanced hydrophilicity, and minimal toxicity. The results of this study clearly highlight the potential of these conjugates as promising small bioactive molecules.<\/jats:p>","DOI":"10.1002\/cbdv.202403440","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T03:20:32Z","timestamp":1742786432000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis, Characterization, Bioevaluation, and Docking Studies of Spiroisatin\u2010Based Hydrazide Conjugates"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6679-0811","authenticated-orcid":false,"given":"Muhammad Imran","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Chemistry Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habiba","family":"Nazir","sequence":"additional","affiliation":[{"name":"Department of Radiological Sciences and Imaging Technology Ibadat International University Islamabad (Pakpattan Campus)  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mross","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Chemical Biology TU Dortmund University  Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9930-858X","authenticated-orcid":false,"given":"Klaus","family":"Jurkschat","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Chemical Biology TU Dortmund University  Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2788-2958","authenticated-orcid":false,"given":"Muhammad Moazzam","family":"Naseer","sequence":"additional","affiliation":[{"name":"Department of Chemistry Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.12.009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.105"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010023\u201000840\u20109"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.02.018"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.02.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/247255522091"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24060"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.01.009"},{"key":"e_1_2_8_10_1","first-page":"1","article-title":"Isatin Derivatives With Several Biological Activities","volume":"6","author":"Grewal A. 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