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In this study, the chemical constituents of WTD were analyzed using ultra\u2010high\u2010performance liquid chromatography\u2010tandem mass spectrometry, identifying a total of 120 compounds, including flavonoids, phenylpropanoids, phenolic acids, alkaloids, etc. Subsequently, network pharmacology analyses revealed that 29 compounds were potential active compounds in WTD for the treatment of RA, as well as 48 core anti\u2010RA targets, including tumor necrosis factor\u2010\u03b1, V\u2010Akt murine thymoma viral oncogene homolog 1, and albumin. Further analysis suggested that WTD treats RA via the phosphoinositide 3\u2010kinase\u2010Akt, mitogen\u2010activated protein kinase, and Ras signaling pathways. Molecular docking analysis of the top five pivotal targets with the core active ingredients demonstrated suitable binding interactions at the active site of target proteins. The significant reduction of nitric oxide levels in lipopolysaccharides\u2010induced RAW264.7 cells validated the anti\u2010inflammation activity of WTD.<\/jats:p>","DOI":"10.1002\/cbdv.202403454","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:39:50Z","timestamp":1741743590000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultra\u2010High\u2010Performance Liquid Chromatography\u2010Tandem Mass Spectrometry Combined With Network Pharmacology to Elucidate the Bioactive Ingredients and Potential Mechanism of Wu\u2010Teng Decoction for Treatment of Rheumatoid Arthritis"],"prefix":"10.1002","volume":"22","author":[{"given":"Wei","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Medicinal Chemistry, School of Pharmaceutical Sciences Guilin Medical University  Guilin 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