{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T17:46:15Z","timestamp":1782668775419,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP\u20100902"],"award-info":[{"award-number":["PIP\u20100902"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT\u20102020\u201003883"],"award-info":[{"award-number":["PICT\u20102020\u201003883"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Diseases caused by trypanosomatid parasites like human African trypanosomiasis (HAT), Chagas disease (CD), leishmaniasis, and malaria are persistent health problems in developing countries that still demand new drug development. The species of the Amaryllidoideae subfamily (Amaryllidaceae) represent a vast source of alkaloids with a wide range of bioactive properties, including antiparasitic effects. The aim of this study was to evaluate the antiparasitic activity of the alkaloids hamayne, 7\u2010hydroxyclivonine, 4\u2010<jats:italic>O<\/jats:italic>\u2010methylnangustine, and candimine against <jats:italic>Trypanosoma brucei rhodesiense<\/jats:italic>, <jats:italic>Trypanosoma cruzi<\/jats:italic>, <jats:italic>Leishmania donovani<\/jats:italic>, and <jats:italic>Plasmodium falciparum<\/jats:italic> parasites. The alkaloids were isolated from the leaves of <jats:italic>Hippeastrum argentinum<\/jats:italic> and <jats:italic>Hippeastrum escoipense<\/jats:italic> using several chromatographic techniques and then identified by GC\u2013MS, UPLC\u2013MS\/MS, and NMR data. The compounds were assessed against different life cycle stages of these four parasites. Furthermore, the cytotoxic activity of the alkaloids against L6 rat skeletal myoblast cells was tested. <jats:italic>P. falciparum<\/jats:italic> was very sensible to 7\u2010hydroxyclivonine. Candimine showed significant antiparasitic activity against all the evaluated parasites, especially <jats:italic>T. b. rhodesiense<\/jats:italic>. Candimine merits deeper research regarding its effect against trypanosomatid parasites as a lead compound for the development of alternative treatments for HAT, CD, and malaria.<\/jats:p>","DOI":"10.1002\/cbdv.202500015","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T19:18:56Z","timestamp":1742325536000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010Trypanosomatid and Antiplasmodial Activities of Alkaloids From <i>Hippeastrum<\/i> Species"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6368-3115","authenticated-orcid":false,"given":"Javier E.","family":"Ortiz","sequence":"first","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Facultad de Ingenier\u00eda Universidad Nacional de San Juan  San Juan Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8895-2799","authenticated-orcid":false,"given":"Mauricio","family":"Pi\u00f1eiro","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Facultad de Ingenier\u00eda Universidad Nacional de San Juan  San Juan Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) CCT CONICET San Juan  San Juan Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1785-7302","authenticated-orcid":false,"given":"Marcel","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute  Allschwil Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3122-1941","authenticated-orcid":false,"given":"Pascal","family":"M\u00e4ser","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute  Allschwil Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-5927","authenticated-orcid":false,"given":"Jaume","family":"Bastida","sequence":"additional","affiliation":[{"name":"Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farm\u00e0cia i Ci\u00e8ncies de l'Alimentaci\u00f3 Universitat de Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4076-3999","authenticated-orcid":false,"given":"Gabriela E.","family":"Feresin","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Facultad de Ingenier\u00eda Universidad Nacional de San Juan  San Juan Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) CCT CONICET San Juan  San Juan Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"WHO \u201cFact Sheets \u201d accessed March 13 2025 https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010022\u201000777\u2010y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010017\u20102204\u20107"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/B978-012373944-5.00211-X","volume-title":"Encyclopedia of Microbiology","author":"Kirchhoff L. V.","year":"2009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.8b00159"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16111416"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2021.100368"},{"key":"e_1_2_9_9_1","first-page":"113","volume-title":"The Alkaloids: Chemistry and Biology","author":"Berkov S.","year":"2020"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0031\u20109422(02)00154\u20101"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010020\u201004171\u20106"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11010144"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154788"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b00785"},{"key":"e_1_2_9_15_1","first-page":"87","volume-title":"The Alkaloids: Chemistry and Biology","author":"Bastida J.","year":"2006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2010.02.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/np100449g"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007270"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000440"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10081913"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.2794"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135556"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/diseases10040090"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20100880\u20105414"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.14522\/darwiniana.2022.102.1066"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1985.tb03772.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0001\u2010706x(97)00079\u2010x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1846"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T19:36:18Z","timestamp":1743104178000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202500015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,27]]},"references-count":27,"alternative-id":["10.1002\/cbdv.202500015"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202500015","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,27]]},"assertion":[{"value":"2025-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202500015"}}