{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:07:38Z","timestamp":1753862858081,"version":"3.41.2"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T00:00:00Z","timestamp":1751155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, we explored an efficient method for synthesizing sulfated <jats:italic>Pleurotus ostreatus<\/jats:italic> polysaccharides (SPOP) with a higher degree of substitution. The structure conformation was characterized and confirmed by the Fourier transform infrared (FT\u2010IR) spectroscopy analysis. A three\u2010factor\u2010three\u2010level Box\u2013Behnken design (BBD) was successfully applied to optimize the SPOP synthesis process. Under the optimal conditions, including a ratio of pyridine to chlorosulfonic acid of 5.10:1, a reaction temperature of 59.92\u00b0C, and a reaction time of 3.05\u00a0h, the predicted maximum degree of sulfate substitution (DS) reached 0.423\u00a0\u00b1\u00a00.006. Furthermore, we evaluated the in vitro radical scavenging abilities and in vivo anti\u2010acute liver injury (anti\u2010ALI) effects of SPOP. The results showed that SPOP demonstrated significantly superior hepatoprotective effects against CCl<jats:sub>4<\/jats:sub>\u2010induced liver damage, specifically by enhancing antioxidant activities both in vitro and in vivo, as well as improving hepatic functions. Our findings suggested that SPOP exhibited significant potential as a natural therapeutic agent for ALI and its associated complications, and contributed to the potential antioxidant capacities.<\/jats:p>","DOI":"10.1002\/cbdv.202500031","type":"journal-article","created":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T17:18:37Z","timestamp":1751217517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization and Hepatoprotective Potential of Sulfated Polysaccharide From <i>Pleurotus ostreatus<\/i>"],"prefix":"10.1002","author":[{"given":"Wen\u2010xin","family":"Xu","sequence":"first","affiliation":[{"name":"College of Life Sciences Shandong Agricultural University  Taian Shandong P. R. 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