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Although VD exhibited weak antibacterial activity (minimum inhibitory concentration [MIC]\u00a0&gt;\u00a0512\u00a0\u00b5g\/mL), it demonstrated potent motility inhibition at sub\u2010inhibitory concentrations, with the highest inhibition observed in swimming (78.13%), twitching (70.96%), and swarming (56.74%) across various strains. Tube assay showed highest inhibition on Day 1 (32.73%) and Day 3 (15.58%) across various strains with VD. Detailed microscopic analysis (light, florescence, and scanning electron microscopy) clearly show that veratraldehyde effectively inhibits biofilm formation in multiple <jats:italic>P. aeruginosa<\/jats:italic> strains. In silico molecular docking and dynamic simulation studies suggest that veratraldehyde may target the PilY protein, a component of Type\u2010IV pili involved in pilus biogenesis, potentially disrupting biofilm formation at a molecular level. In silico pharmacokinetic analysis such as absorption, distribution, metabolism, and excretion (ADME) analysis indicates favorable properties (e.g., bioavailability, solubility, drug likeness, high gastrointestinal (GI) absorption, and skin permeability), making veratraldehyde a promising candidate for anti\u2010biofilm therapeutic development. These results highlight its potential as a natural alternative to conventional antibiotics in combating <jats:italic>P. aeruginosa<\/jats:italic> biofilm associated infections.<\/jats:p>","DOI":"10.1002\/cbdv.202500074","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T18:50:51Z","timestamp":1748631051000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Veratraldehyde Inhibits Motility Phenotypes and Targets Biofilm Formation of <i>Pseudomonas aeruginosa<\/i>: Insights From Computational and Experimental Studies"],"prefix":"10.1002","author":[{"given":"Monish","family":"Bisen","sequence":"first","affiliation":[{"name":"School of Biotechnology Faculty of Applied Sciences and Biotechnology Shoolini University  Solan Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0692-0137","authenticated-orcid":false,"given":"Lokender","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Biotechnology Faculty of Applied Sciences and Biotechnology Shoolini University  Solan Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01056-1"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00477-5"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2021.107734"},{"key":"e_1_2_14_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12088-024-01221-w","article-title":"Current Strategies for Combating Biofilm\u2010Forming Pathogens in Clinical Healthcare\u2010Associated Infections","volume":"64","author":"Biswas R.","year":"2024","journal-title":"Indian Journal of Microbiology"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880502801106"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880502801103"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0235093"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1705814"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.15698\/mic2021.02.741"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.glmedi.2024.100081"},{"key":"e_1_2_14_12_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1080\/1040841X.2024.2321480","article-title":"Current Developments and Prospects of the Antibiotic Delivery Systems","volume":"51","author":"Kharga K.","year":"2024","journal-title":"Critical Reviews in Microbiology"},{"key":"e_1_2_14_13_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.851784","article-title":"Pseudomonas aeruginosa Is More Tolerant Under Biofilm Than Under Planktonic Growth Conditions: A Multi\u2010Isolate Survey","volume":"12","author":"Th\u00f6ming J. 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