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Baicalein is a flavonoid compound with anti\u2010inflammatory properties that exerts a protective effect on blood vessels. However, whether baicalein can inhibit vascular damage induced by NH<jats:sub>2<\/jats:sub>\u2010NPs remains to be investigated. In this study, cell viability assay, fluorescence staining observation, and western blot techniques were used to evaluate the mechanisms after NH<jats:sub>2<\/jats:sub>\u2010NPs and baicalein exposure. The results showed that baicalein at concentrations of 12.5, 25, and 50 \u00b5M effectively inhibited the toxicity induced by NH<jats:sub>2<\/jats:sub>\u2010NPs. Besides, baicalein reduced reactive oxygen species levels to 226.63 \u00b1\u00a025.65%, 168.13 \u00b1\u00a014.45%, and 124.70 \u00b1\u00a018.04% at 12.5, 25, and 50 \u00b5M, respectively. The results of Hoechst\/propidium iodide (PI) staining showed that 50 \u00b5M baicalein reduced the PI\u2010positive rate to 12.42 \u00b1\u00a01.16%. Additionally, co\u2010incubation with baicalein significantly reduced the expression of proteins in the pyroptosis pathway, including NLRP3, Cleaved Caspase\u20101, ASC, and Gasdermin D, effectively inhibiting the activation of pyroptosis induced by NH<jats:sub>2<\/jats:sub>\u2010NPs in human umbilical vein endothelial cells (HUVECs). This study elucidated the mechanism by which baicalein inhibited NH<jats:sub>2<\/jats:sub>\u2010NPs\u2010induced pyroptosis in HUVECs, providing a reference for the prevention of vascular injury and utilization of natural product resources.<\/jats:p>","DOI":"10.1002\/cbdv.202500085","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T23:16:06Z","timestamp":1740006966000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Baicalein Inhibited\u00a0Amino\u2010modified Polystyrene Nanoplastics Induced Human Umbilical Vein Endothelial Cells Pyroptosis by Reducing\u00a0the Expression\u00a0of NLRP3\/Caspase\u20101\/Gasdermin D Pathway\u2010related Proteins"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8776-4892","authenticated-orcid":false,"given":"Junliang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, School of Life Science Liaoning University Shenyang P. 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