{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:07:09Z","timestamp":1753862829805,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In vitro anticancer and antimicrobial activities of 24 pyridine thiosemicarbazone (PTSC) derivatives (<jats:bold>3a\u20133x<\/jats:bold>), synthesized via a two\u2010step process starting from hydrazine hydrate and carbon disulfide, have demonstrated promising results. Five compounds <jats:bold>3<\/jats:bold>\u00a0<jats:bold>g<\/jats:bold>, <jats:bold>3<\/jats:bold>\u00a0<jats:bold>h<\/jats:bold>, <jats:bold>3v<\/jats:bold>, <jats:bold>3w<\/jats:bold>, and <jats:bold>3x<\/jats:bold> exhibited significant anticancer activity at a concentration of 10 \u00b5M against several cancer cell lines, including leukemia (CCRF\u2010CEM and MOLT\u20104), melanoma (SK\u2010MEL\u20102 and SK\u2010MEL\u20105), and breast cancer (MDA\u2010MB\u2010468), with growth percentage values ranging from \u201011.61% to \u201075.49%. Among these, compound <jats:bold>3w<\/jats:bold> displayed exceptional anticancer efficacy against the renal cancer cell line UO\u201031, with a GI\u2085\u2080 value of 0.57 \u00b5M, while showing no cytotoxicity toward normal cells (lethal concentration 50% &gt; 100 \u00b5M). Further in vitro evaluations using ovarian (IGROV1), colon (HCC\u20102998), and melanoma (MALME\u20103\u00a0M) cancer cell lines revealed that compounds <jats:bold>3<\/jats:bold>\u00a0<jats:bold>g<\/jats:bold>, <jats:bold>3<\/jats:bold>\u00a0<jats:bold>h<\/jats:bold>, and <jats:bold>3w<\/jats:bold> exhibited GI\u2085\u2080 values ranging from 2.00 to 4.89 \u00b5M, supporting their cytotoxic potential. Notably, compounds <jats:bold>3<\/jats:bold>\u00a0<jats:bold>g<\/jats:bold>, <jats:bold>3<\/jats:bold>\u00a0<jats:bold>h<\/jats:bold>, <jats:bold>3w<\/jats:bold>, and <jats:bold>3x<\/jats:bold> were found to be more potent than the reference drug, <jats:bold>Sunitinib<\/jats:bold>. These findings highlight the therapeutic potential of PTSC derivatives and underscore their promise as lead compounds in anticancer drug development. Additionally, all PTSC derivatives were evaluated for antimicrobial efficacy against five different Gram\u2010positive and Gram\u2010negative bacterial strains and two fungal pathogens at a concentration of 32 \u00b5g\/mL. Among them, compound <jats:bold>3q<\/jats:bold> exhibited the highest growth inhibition (GI) of 97.63% against <jats:italic>Acinetobacter baumannii<\/jats:italic>, without exhibiting any toxicity toward human embryonic kidney (HEK\u2010293) cells and human red blood cells at concentrations ranging from 32 to 0.25 \u00b5g\/mL. Furthermore, compounds <jats:bold>3o<\/jats:bold>, <jats:bold>3k<\/jats:bold>, and <jats:bold>3x<\/jats:bold> demonstrated in vitro GI percentages of 53.24%, 77.67%, and 95.77%, respectively, against <jats:italic>Candida albicans<\/jats:italic>. These results support the potential of PTSC derivatives as multifunctional therapeutic agents with both anticancer and antimicrobial properties.<\/jats:p>","DOI":"10.1002\/cbdv.202500202","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T17:50:59Z","timestamp":1749145859000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pyridine Thiosemicarbazones as Anticancer and Antimicrobial Agents: A Promising Class of Low\u2010Toxicity Therapeutics"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6435-5271","authenticated-orcid":false,"given":"Navnath Ramdas","family":"Zaware","sequence":"first","affiliation":[{"name":"Department of Chemistry PDEA's Baburaoji Gholap College  Pune India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramakant Asaram","family":"Kardile","sequence":"additional","affiliation":[{"name":"Department of Chemistry B.P.H.E. Society's Ahmednagar College  Ahmednagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-1013","authenticated-orcid":false,"given":"Sangeeta Vijay","family":"Jagtap","sequence":"additional","affiliation":[{"name":"Department of Chemistry PDEA's Baburaoji Gholap College  Pune India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.3322\/caac.21332"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.11648\/j.jddmc.20150101.11"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1155\/2020\/2960165"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1016\/j.ejmech.2019.03.027"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1124\/mol.111.071324"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1158\/0008\u20105472.CAN\u201008\u20101437"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.18632\/oncotarget.24844"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1073\/pnas.0604979103"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1590\/s2175\u201097902019000118218"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_1","DOI":"10.1016\/j.bmcl.2009.03.093"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.3390\/ma15165513"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.3390\/ph16091267"},{"doi-asserted-by":"publisher","key":"e_1_2_8_14_1","DOI":"10.1007\/s11030\u2010019\u201010021\u20100"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.3390\/molecules15020988"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.1016\/j.bmcl.2004.03.069"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.1016\/j.ejmech.2011.07.033"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1007\/s43440\u2010021\u201000221\u20107"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.1016\/j.ejmech.2014.05.001"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.1016\/j.bmcl.2017.04.024"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_1","DOI":"10.1016\/j.bmcl.2006.01.048"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1016\/j.arabjc.2020.11.020"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1002\/ardp.202000267"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.1002\/cbdv.202100504"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.1016\/j.bioorg.2020.103660"},{"doi-asserted-by":"publisher","key":"e_1_2_8_26_1","DOI":"10.1016\/j.bmcl.2024.130079"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1002\/jhet.4370"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1186\/s13065\u2010018\u20100469\u20103"},{"doi-asserted-by":"publisher","key":"e_1_2_8_29_1","DOI":"10.3390\/molecules21010003"},{"doi-asserted-by":"publisher","key":"e_1_2_8_30_1","DOI":"10.1021\/acsinfecdis.5b00044"},{"doi-asserted-by":"publisher","key":"e_1_2_8_31_1","DOI":"10.1002\/cmdc.201600182"},{"doi-asserted-by":"publisher","key":"e_1_2_8_32_1","DOI":"10.3390\/molecules23112913"},{"doi-asserted-by":"publisher","key":"e_1_2_8_33_1","DOI":"10.1016\/j.bmc.2015.01.036"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T17:51:00Z","timestamp":1749145860000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202500202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,5]]},"references-count":32,"alternative-id":["10.1002\/cbdv.202500202"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202500202","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"type":"print","value":"1612-1872"},{"type":"electronic","value":"1612-1880"}],"subject":[],"published":{"date-parts":[[2025,6,5]]},"assertion":[{"value":"2025-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00202"}}