{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:10:29Z","timestamp":1791648629824,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A new class of triazolo[3,4\u2010<jats:italic>b<\/jats:italic>][1,3,4]thiadiazines has been developed by combining thiocarbohydrazide and ethyl cyanoacetate, resulting in the formation of a triazole\u20103\u2010thione derivative. Afterwards, the compound underwent a reaction with 2\u2010bromoacetamide derivative and chloroacetic acid to yield the corresponding triazolo\u2010thiadiazine derivatives, 6\u2010amino\u20105\u2010(aminomethyl)\u20103\u2010methyl\u2010<jats:italic>N<\/jats:italic>\u2010(5\u2010methylthiazol\u20102\u2010yl)\u20105<jats:italic>H<\/jats:italic>\u2010[1,2,4]triazolo[3,4\u2010<jats:italic>b<\/jats:italic>][1,3,4]thiadiazine\u20107\u2010carboxamide derivative and 5\u2010(aminomethyl)\u20103\u2010methyl\u20105<jats:italic>H<\/jats:italic>\u2010[1,2,4]triazolo[3,4\u2010b][1,3,4]\u2010 thiadiazin\u20106(7<jats:italic>H<\/jats:italic>)\u2010one derivative which can serve as a precursor in subsequent processes. Furthermore, when \u03b1\u2010bromo ketones were treated with triazole derivative, which was prepared by mixing thiocarbohydrazide with 4\u2010hydroxybenzoic acid, 5<jats:italic>H<\/jats:italic>\u2010[1,2,4]triazolo[3,4\u2010<jats:italic>b<\/jats:italic>][1,3,4]thiadiazin\u20103\u2010yl)phenol derivatives were produced. To identify each of the produced compounds, advanced spectroscopic techniques such as mass spectrometry, proton nuclear magnetic resonance (<jats:sup>1<\/jats:sup>H NMR), carbon\u201013 NMR (<jats:sup>13<\/jats:sup>C NMR), and Fourier\u2010transform infrared spectroscopy were utilized. Most of the newly generated compounds showed significant inhibitory effects in vitro against the acetylcholinesterase enzyme, making them potential candidates for treating Alzheimer's disease. Two compounds in particular, triazole\u20103\u2010thione derivative, and 4\u2010(6\u2010(benzofuran\u20102\u2010yl)\u20105<jats:italic>H<\/jats:italic>\u2010[1,2,4]triazolo[3,4\u2010<jats:italic>b<\/jats:italic>][1,3,4]thiadiazin\u20103\u2010yl)phenol showed the most promising therapeutic potential with IC<jats:sub>50<\/jats:sub> values of 14.58 \u00b1 0.45 and 22.53 \u00b1 0.68\u00a0\u00b5g\/mL, respectively.<\/jats:p>","DOI":"10.1002\/cbdv.202500260","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T19:19:56Z","timestamp":1742239196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Synthesizing Unique Triazolo[3,4\u2010b][1,3,4]Thiadiazines as Effective Anti\u2010Alzheimer's Medications: An In Vitro Study"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1586-6910","authenticated-orcid":false,"given":"Marwa M.","family":"Fouad","sequence":"first","affiliation":[{"name":"Basic Science Department Modern Academy for Engineering &amp; Technology  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1957-8377","authenticated-orcid":false,"given":"Ahmed M.","family":"Fouad","sequence":"additional","affiliation":[{"name":"Department of Physics Temple University  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2586-9653","authenticated-orcid":false,"given":"Amr Mohamed","family":"Fouad","sequence":"additional","affiliation":[{"name":"Neurology Department Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28010021"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527313666141023141545"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2016.10.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301\u20100082(02)00079\u20105"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2016.07.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm7009364"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.71.5.589"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/15701808113109990034"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.farmac.2004.07.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/157017861010131126115448"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2004.09.072"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2011.12.120"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01498"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2010.496506"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.01.010"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/idr.s81416"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2018.02.002"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2022.01.462"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/00397911.2012.711883"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570193X17999200820163532"},{"issue":"2","key":"e_1_2_8_22_1","first-page":"39","article-title":"Al\u2010Azhar","volume":"62","author":"Omar A. 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