{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T07:18:28Z","timestamp":1780557508571,"version":"3.54.1"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Cheilocostus speciosus<\/jats:italic> (J. Koenig) C. Specht, commonly known as \u201ccrepe\u2010ginger,\u201d is a traditional plant with edible flowers utilized in folk medicine. This study uses crepe\u2010ginger flowers to assess their hepatoprotective effects through the management of oxidative stress. <jats:italic>C. speciosus<\/jats:italic> flower's methanolic extract (CSF\u2010ME) was subjected to in vitro antioxidant effects were evaluated using 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) and 2,2\u2032\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) (ABTS) and in vivo by catalase (CAT) assays which ameliorated CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic injury evident by histopathological analysis. The chemical assay was evaluated via phytochemical screening and gas chromatography\u2013tandem mass spectrometry (GC\u2013MS\/MS) analysis followed by in silico studies. The antioxidant assay DPPH (IC<jats:sub>50<\/jats:sub>\u00a0=\u00a0179.36\u00a0\u00b5g\/mL) and ABTS (IC<jats:sub>50<\/jats:sub>\u00a0=\u00a0198.27\u00a0\u00b5g\/mL) showed remarkable scavenging activity. Hepatotoxicity experiments demonstrated that CSF\u2010ME improved liver function by positively regulating AST, ALT, ALP, bilirubin, creatinine, LDL, CHO, TG, HDL, and catalase levels. Besides, histopathological analysis revealed normal hepatocyte integrity and microstructures after treatment. Besides, phytochemical screening revealed prospective phytochemical groups, while GC\u2013MS\/MS analysis recognized 40 compounds, resulting in auspicious outcomes employing computer\u2010aided studies. The findings indicated that the CSF\u2010ME possesses promising hepatoprotective and antioxidant prospects, which demand further extensive research to develop novel lead compounds from this natural source.<\/jats:p>","DOI":"10.1002\/cbdv.202500261","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T11:54:48Z","timestamp":1742385288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Amelioration of Hepatic Injury Through Oxidative Stress Management Employing Methanolic Extract of Crepe\u2010Ginger (<i>Cheilocostus speciosus<\/i> (J. Koenig) C. Specht) Flower"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-899X","authenticated-orcid":false,"given":"Mahathir","family":"Mohammad","sequence":"first","affiliation":[{"name":"Department of Chemistry Chittagong University of Engineering &amp; Technology  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7271-4219","authenticated-orcid":false,"given":"Fahmida Tasnim","family":"Richi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"},{"name":"Department of Pharmacy University of Asia Pacific  74\/A, Green Road, Farmgate Dhaka 1215 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Arafat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology University of Science and Technology  Foy\u2019s Lake Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pair Ahmed","family":"Jiko","sequence":"additional","affiliation":[{"name":"Department of Chemistry Chittagong University of Engineering &amp; Technology  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5980-3877","authenticated-orcid":false,"given":"Mohammad Rashedul","family":"Haque","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8759-9279","authenticated-orcid":false,"given":"Md. Hemayet","family":"Hossain","sequence":"additional","affiliation":[{"name":"Chemical Research Division, BCSIR Dhaka Laboratories Bangladesh Council of Scientific and Industrial Research  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Sakhaoyat","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Chemistry Chittagong University of Engineering &amp; Technology  Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0585-8500","authenticated-orcid":false,"given":"Sania","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Abdullah","family":"Taher","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"},{"name":"Bangladesh Reference Institute for Chemical Measurements (BRiCM)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-2278","authenticated-orcid":false,"given":"Safaet","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"},{"name":"Chemical Research Division, BCSIR Dhaka Laboratories Bangladesh Council of Scientific and Industrial Research  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,12]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"1","volume-title":"The Liver: Biology and Pathobiology","author":"Nagy P.","year":"2020"},{"issue":"12","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.3109\/00365529609036901","article-title":"Animal Models of Liver Fibrosis","volume":"31","author":"Wu J.","year":"1996","journal-title":"Scandinavian Journal of Gastroenterology"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113840"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712803341520"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161125942"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ejphar.2017.11.036","article-title":"17\u03b2\u2010Estradiol Suppresses Carboxylesterases by Activating c\u2010Jun\/AP\u20101 Pathway in Primary Human and Mouse Hepatocytes","volume":"819","author":"Wu L.","year":"2018","journal-title":"European Journal of Pharmacology"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i16.2912"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13237\u2010022\u201000405\u20103"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.30495\/tpr.2021.680480"},{"issue":"2","key":"e_1_2_11_11_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1054\/plef.1999.0128","article-title":"Antioxidant Activity of Selected Indian Spices","volume":"62","author":"Shobana S.","year":"2000","journal-title":"Prostaglandins, Leukotrienes and Essential Fatty Acids"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.4103\/2231-4040.70519","article-title":"Assessment of Anti\u2010Nociceptive Efficacy of Costus speciosus Rhizome in Swiss Albino Mice","volume":"1","author":"Bhattacharya S.","year":"2010","journal-title":"Journal of Advanced Pharmaceutical Technology and Research"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201012\u201013"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.sajb.2015.01.012","article-title":"Costus speciosus and Coccinia grandis: Traditional Medicinal Remedies for Diabetes","volume":"98","author":"Waisundara V.","year":"2015","journal-title":"South African Journal of Botany"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21347"},{"issue":"1","key":"e_1_2_11_16_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ygeno.2011.03.011","article-title":"Global Analysis of Gene\u2010Level MicroRNA Expression in Arabidopsis Using Deep Sequencing Data","volume":"98","author":"Yang X.","year":"2011","journal-title":"Genomics"},{"issue":"1","key":"e_1_2_11_17_1","first-page":"15","article-title":"In Vivo and In Vitro Initiatives for the Examination of Pharmacological Properties of Brassaiopsis hainla Leaves","volume":"9","author":"Zishan S. 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