{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:40:34Z","timestamp":1773013234561,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diseases resulting from bacterial invasion have consistently posed a substantial threat to public health and safety. To address this problem, this study aimed to discover novel chemical structures with antibacterial activity by using flavonols as lead compounds. Through structural modifications, we introduced various\n                    <jats:italic>N<\/jats:italic>\n                    \u2010substituted piperazine compounds to synthesize ten previously unreported compounds. Subsequently, the antibacterial activities of these compounds against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    were evaluated. Compound\n                    <jats:bold>2<\/jats:bold>\n                    <jats:bold>g<\/jats:bold>\n                    was identified as the most potent compound, exhibiting minimum inhibitory concentration (MIC) values of 6.25 \u00b5g\/mL against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and 25 \u00b5g\/mL against\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Additionally, compound\n                    <jats:bold>2g<\/jats:bold>\n                    effectively inhibited the growth of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    , with inhibition zones measuring 16\u00a0mm and 15\u00a0mm, respectively. At a concentration of 8 \u00d7 MIC, the bacterial counts of both strains were reduced by 1.75 and 0.72 logCFU\/mL within 60 min. Furthermore, compound\n                    <jats:bold>2g<\/jats:bold>\n                    significantly inhibited the formation of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    biofilms. Molecular docking studies suggested that the staphylococcal accessory regulator might be its potential target. This study provides valuable insights into the antibacterial activity of flavonoids and offers a scientific foundation for the development of novel antibacterial agents.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500272","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T15:38:31Z","timestamp":1751384311000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis and Evaluation of the Antibacterial Activity of\n                    <i>N<\/i>\n                    \u2010Substituted Piperazine Flavonol Derivatives"],"prefix":"10.1002","volume":"22","author":[{"given":"Jin","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Ming","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si\u2010Jiao","family":"Cui","sequence":"additional","affiliation":[{"name":"Shenyang Food and Drug Inspection Institute  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Li","family":"Sui","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4284-7990","authenticated-orcid":false,"given":"Tian","family":"Luan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shenyang Medical College  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy955"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28073118"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17091241"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S407891"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S455979"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.08.071"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2015.1708"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.09.015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2015.12.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms131216785"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200600173"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102010"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10123-022-00245-w"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27082494"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-17-214"},{"key":"e_1_2_9_17_1","first-page":"10","author":"Wen Q.\u2010H.","year":"2021","journal-title":"Foods"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmx112"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-78379-y"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e03013"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2023.2237701"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm5004599"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/fcp.12408"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.12.015"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23112859"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm8008629"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510439103"}],"container-title":["Chemistry &amp; 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