{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:07:33Z","timestamp":1753862853533,"version":"3.41.2"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The advancement of drug delivery systems (DDSs) for controlled and targeted release needs further development to provide efficient cancer treatment. In this research, nanoliposomes (Lip) containing <jats:italic>Cassia alata<\/jats:italic> extract (CA) coated with chitosan\u2013folate (Chi\u2013FA) have been successfully synthesized as a DDS to breast cancer cells. Anticancer compounds were extracted from CA, and the effects of several parameters, such as ethanol concentration, ultrasonication time, and simplicia:solvent ratio, were analyzed using the response surface methodology (RSM). The successful conjugation of Chi\u2013FA was observed using UV\u2013Vis, FTIR, and XRD. The formation of Lip, CA\u2010loaded nanoliposomes (Lip\u2010CA), and Chi\u2013FA coated Lip\u2010CA (Lip\u2010CA@Chi\u2013FA) was validated using ATR\u2010FTIR and TEM. Drug release studies show that nanoliposomes are selective in cancer cell conditions. The use of chitosan folate\u2010conjugated nanoliposome encapsulation can deliver the extract more targeted to T47D breast cancer cells. The cytotoxicity test showed that Lip\u2010CA@Chi\u2013FA exhibited an IC\u2085\u2080 value of 53.46\u00a0\u00b5g\/mL against T47D cells, indicating moderate anticancer activity and improved delivery efficiency compared to non\u2010encapsulated extract. In silico studies to predict pharmacokinetics and molecular docking simulations of the main compounds of CA support the experimental findings.<\/jats:p>","DOI":"10.1002\/cbdv.202500276","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T18:51:33Z","timestamp":1748631093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Silico and In Vitro Evaluation of Chitosan\u2010Folate Coated Nanoliposomes Loaded With <i>Cassia alata<\/i> Leaf Extract for Targeted Breast Cancer Therapy"],"prefix":"10.1002","author":[{"given":"Setyanto Arief","family":"Wiedagdo","sequence":"first","affiliation":[{"name":"Department of Chemistry Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Kusumaningrum","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjelika Putri","family":"Febriyanti","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fasha Putri","family":"Arkhani","sequence":"additional","affiliation":[{"name":"Department of Medicine Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rizky Eka","family":"Putera","sequence":"additional","affiliation":[{"name":"Department of Medicine Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fajar Rakhman","family":"Wibowo","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8813-3649","authenticated-orcid":false,"given":"Maulidan","family":"Firdaus","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universitas Sebelas Maret  Surakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174287"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21208"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.14.110"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11412"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0111-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0341-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1186\/s12906-023-04198-z","article-title":"The Anticancer Potential of Chemical Constituents of Moringa oleifera Targeting CDK\u20102 Inhibition in Estrogen Receptor Positive Breast Cancer Using In\u2010Silico and In Vitro Approaches","volume":"23","author":"Sultan R.","year":"2023","journal-title":"BMC Complementary Medicine and Therapies"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2017.1380655"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2016.11.005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4103\/IJPC.IJPC_82_17","article-title":"Quality of Life Among Cancer Patients","volume":"23","author":"Nayak M. 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