{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:43:43Z","timestamp":1790027023354,"version":"4.0.1"},"reference-count":65,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>There is a need to find efficient treatment for toxoplasmosis, a global disease with a public health concern, as the used therapeutics have limited effectiveness. Beta\u2010glucan (\u03b2\u2010glucan), a natural polysaccharide, has anti\u2010inflammatory, immunomodulatory, and anti\u2010infective activities against bacteria, viruses, and parasites. We aimed to investigate the therapeutic efficacy of \u03b2\u2010glucan on toxoplasmosis using an in silico study and an in vivo mice model. The \u03b2\u2010glucan drug\u2010likeness characteristics were evaluated by molecular docking simulations, Lipinski's RO5 filter analysis, and the computational quantitative structural\u2010activity relationships\/absorption, distribution, metabolism, excretion, and toxicity predictive characteristics compared to spiramycin, an anti\u2010toxoplasmosis drug. Sixty albino mice were divided into two divisions, immunocompetent and immunosuppressed. Each division contained five groups; healthy, diseased, spiramycin\u2010treated, \u03b2\u2010glucan\u2010treated, and both \u03b2\u2010glucan and spiramycin\u2010treated groups. Parasitological, histopathological examinations, and immunological analysis were detected. \u03b2\u2010glucan docked complexes demonstrated H\u2010bonding and hydrophobic interactions towards their promising anti\u2010toxoplasmosis targets compared to the spiramycin. The treated mice revealed a statistically significant reduction (<jats:italic>p<\/jats:italic> &lt; 0.001) in the parasite burden. Regarding immunocompetent and immunosuppressed division, the group that was treated with both \u03b2\u2010glucan and spiramycin, demonstrated the greatest outcomes, with 71% and 66% efficacy, respectively. It is concluded that \u03b2\u2010glucan shows promising potential as an anti\u2010toxoplasmosis candidate by potentiating the spiramycin effect and modulating the immunological response.<\/jats:p>","DOI":"10.1002\/cbdv.202500370","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T15:50:23Z","timestamp":1744041023000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigating Therapeutic Potential of Beta\u2010Glucan on Experimental Toxoplasmosis in Immunocompetent and Immunocompromised Mice: In Silico and in Vivo Studies"],"prefix":"10.1002","author":[{"given":"Asmaa A.","family":"Aboul\u2010Fotouh","sequence":"first","affiliation":[{"name":"Zoology Department Faculty of Science for Girls Al\u2010Azhar University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faten A.","family":"Elfeky","sequence":"additional","affiliation":[{"name":"Zoology Department Faculty of Science for Girls Al\u2010Azhar University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahed R.","family":"Omar","sequence":"additional","affiliation":[{"name":"Zoology Department Faculty of Science for Girls Al\u2010Azhar University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek","family":"Aboushousha","sequence":"additional","affiliation":[{"name":"Department of Pathology Theodor Bilharz Research Institute  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0082-9412","authenticated-orcid":false,"given":"Eman S.","family":"El\u2010Wakil","sequence":"additional","affiliation":[{"name":"Department of Parasitology Theodor Bilharz Research Institute  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.2174\/0118749445281387240202094637","article-title":"A Comprehensive Review of Toxoplasmosis: Serious Threat to Human Health","volume":"17","author":"Khairullah A. 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