{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:47:55Z","timestamp":1792511275758,"version":"4.5.0"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nonylphenol (NP) is an environmental contaminant known for its endocrine\u2010disrupting properties, which can lead to male infertility. This study investigates the therapeutic potential of <jats:italic>Cucurbita maxima<\/jats:italic> Duch. seed extract (CMSE) in ameliorating NP\u2010induced reproductive toxicity in male mice. Gas chromatography\u2010mass spectrometry was used to identify the bioactive non\u2010polar compounds of CMSE responsible for its activity. The binding affinity of these compounds with endothelial nitric oxide synthase, the primary neurotransmitter that triggers penile erection through the nitric oxide (NO)\u2010cyclic guanosine monophosphate pathway using AutoDock Vina was investigated. Adult male mice were divided into six groups (<jats:italic>n<\/jats:italic> = 8) and infertility was induced by administering NP (50 mg\/kg b.wt.) orally with olive oil for 35 days. Two doses of CMSE (250 and 500 mg\/kg b.wt.) were administered, and blood samples were collected for serum NO analysis. Different sexual activities and fertility study was also done to investigate the reproductive performance. The results showed that NP significantly decreased serum NO levels, sexual activity and reproductive performance of mice whereas CMSE helped to mitigate these negative effects.<\/jats:p>","DOI":"10.1002\/cbdv.202500480","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T16:33:12Z","timestamp":1744043592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Therapeutic Potential of <i>Cucurbita maxima<\/i> Duch. Seed Extract in Ameliorating Nonylphenol\u2010Induced Reproductive Toxicity in Male Mice: In\u2010Vivo and In\u2010Silico Approaches"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0744-9807","authenticated-orcid":false,"given":"Taslema","family":"Begum","sequence":"first","affiliation":[{"name":"Department of Zoology Gauhati University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Queen","family":"Saikia","sequence":"additional","affiliation":[{"name":"Department of Zoology Mangaldai College  Mangaldai Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jogen Chandra","family":"Kalita","sequence":"additional","affiliation":[{"name":"Department of Zoology Gauhati University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"e_1_2_14_2_1","volume-title":"Reproduction in Farm Animals","author":"Hafez E. 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