{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:07:34Z","timestamp":1781179654870,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007288","name":"Mahasarakham University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n Phytochemical investigation of the methanol extract of\n Clausena excavata<\/jats:italic>\n roots led to the isolation of six pyranocoumarin derivatives, including xanthyletin (\n 1<\/jats:bold>\n ), kinocoumarin (\n 2<\/jats:bold>\n ), clausarin (\n 3<\/jats:bold>\n ), nordentatin (\n 4<\/jats:bold>\n ), dentatin (\n 4a<\/jats:bold>\n ), and citrusarin A (\n 5<\/jats:bold>\n ), and three carbazole alkaloids, that is, heptaphylline (\n 6<\/jats:bold>\n ), 7\u2010methoxymukonal (\n 7<\/jats:bold>\n ), and clausine K (\n 8<\/jats:bold>\n ). Seven new semisynthetic analogs of compounds\n 2<\/jats:bold>\n ,\n 3,<\/jats:bold>\n and\n 4<\/jats:bold>\n (\n 2a<\/jats:bold>\n ,\n 3b<\/jats:bold>\n \u2013\n 3d<\/jats:bold>\n , and\n 4b<\/jats:bold>\n \u2013\n 4d<\/jats:bold>\n ) were prepared through methylation, etherification, and basic hydrolysis for biological assays. Compounds\n 2<\/jats:bold>\n ,\n 6<\/jats:bold>\n ,\n 3b<\/jats:bold>\n ,\n 3c<\/jats:bold>\n ,\n 4b,<\/jats:bold>\n and\n 4c<\/jats:bold>\n displayed stronger \u03b1\u2010glucosidase inhibitory activity than the positive control, acarbose (IC\n 50<\/jats:sub>\n 391.47\u00a0\u00b5M), with IC\n 50<\/jats:sub>\n values in the range of 32.89\u201392.55\u00a0\u00b5M. Molecular docking suggested that these compounds bind near the enzyme's active site via van der Waals forces, hydrogen bonds, and hydrophobic interactions. Compound\n 3<\/jats:bold>\n showed the strongest nitric oxide (NO) inhibitory activity (IC\n 50<\/jats:sub>\n \u00a0=\u00a027.95\u00a0\u00b5M), followed by\n 3d<\/jats:bold>\n (IC\n 50<\/jats:sub>\n \u00a0=\u00a033.62\u00a0\u00b5M) and\n 4<\/jats:bold>\n (IC\n 50<\/jats:sub>\n \u00a0=\u00a035.41\u00a0\u00b5M). For the two compounds tested for cytotoxicity against A549 cells,\n 3b<\/jats:bold>\n (IC\n 50<\/jats:sub>\n \u00a0=\u00a011.71\u00a0\u00b5M) was more potent than cisplatin (IC\n 50<\/jats:sub>\n \u00a0=\u00a021.56\u00a0\u00b5M), whereas\n 4b<\/jats:bold>\n (IC\n 50<\/jats:sub>\n \u00a0=\u00a022.39\u00a0\u00b5M) showed similar activity to cisplatin. Drug\u2010likeness and pharmacokinetic properties of the isolated and semisynthetic compounds were also evaluated.\n <\/jats:p>","DOI":"10.1002\/cbdv.202500510","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T18:48:37Z","timestamp":1753728517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["\u03b1\u2010Glucosidase and Nitric Oxide Inhibitory Activities of Pyranocoumarins and Carbazoles From the Roots of\n Clausena excavata<\/i>\n and the Semisynthetic Analogs of Clausarin and Nordentatin"],"prefix":"10.1002","volume":"22","author":[{"given":"Thanatcha","family":"Samsee","sequence":"first","affiliation":[{"name":"Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH\u2010CIC) Faculty of Science Mahasarakham University, Khamriang, Kantarawichai Maha Sarakham 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