{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:12:40Z","timestamp":1792033960927,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A series of thiazole\u2013piperazine sulphonamide hybrids (\n                    <jats:bold>8a\u2013k<\/jats:bold>\n                    ) were synthesized, characterized and subsequently tested on Alzheimer's disease (AD) targets, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and the ABTS radical, to assess their effectiveness. Three of the target analogues\n                    <jats:bold>8c<\/jats:bold>\n                    ,\n                    <jats:bold>8e<\/jats:bold>\n                    and\n                    <jats:bold>8g<\/jats:bold>\n                    exhibited augmented inhibition on AChE with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 2.52\u00a0\u00b1\u00a00.92, 2.99\u00a0\u00b1\u00a00.01 and 2.14\u00a0\u00b1\u00a00.02\u00a0\u00b5M, respectively. These analogues also showed strong inhibition selectivity against AChE over BChE. Furthermore, the congeners\n                    <jats:bold>8d<\/jats:bold>\n                    ,\n                    <jats:bold>8f<\/jats:bold>\n                    ,\n                    <jats:bold>8h<\/jats:bold>\n                    and\n                    <jats:bold>8i<\/jats:bold>\n                    had remarkable ABTS radical scavenging properties as their IC\n                    <jats:sub>50<\/jats:sub>\n                    values were in the range of 0.05\u00a0\u00b1\u00a00.07\u20130.99\u00a0\u00b1\u00a00.12\u00a0\u00b5M. A study of the kinetics of inhibition of AChE for active analogue\n                    <jats:bold>8g<\/jats:bold>\n                    revealed a mixed type of inhibition. From the molecular docking experiments, it was clear that the compounds\n                    <jats:bold>8c<\/jats:bold>\n                    ,\n                    <jats:bold>8e<\/jats:bold>\n                    and\n                    <jats:bold>8g<\/jats:bold>\n                    were placed optimally within the active site of AChE by interacting with both the catalytic active site (CAS) and the peripheral anionic site (PAS) and served as evidence for mixed type of inhibition. Molecular dynamics (MD) simulations of these docked complexes indicated that the root\u2010mean\u2010square deviation (RMSD) of the complexes stabilized below 4\u00a0\u00c5. Among the ligands,\n                    <jats:bold>8c<\/jats:bold>\n                    exhibited the highest stability, with an RMSD of 3.0\u00a0\u00b1\u00a00.30\u00a0\u00c5, compared to\n                    <jats:bold>8e<\/jats:bold>\n                    (3.77\u00a0\u00b1\u00a00.38\u00a0\u00c5) and\n                    <jats:bold>8g<\/jats:bold>\n                    (3.50\u00a0\u00b1\u00a00.52\u00a0\u00c5) in complex with human AChE (hAChE). Furthermore, in silico ADMET prediction studies revealed that the targeted analogues satisfied all the characteristics of central nervous system (CNS) acting drugs. Finally, these active compounds are determined to be nontoxic and highly neuroprotective against H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced cell death in SK\u2010N\u2010SH cell lines. These results inferred that the thiazole\u2013piperazine sulphonamide derivatives have the potential to serve as a valuable molecular template for further in vitro and in vivo assessments in the context of the development of anti\u2010AD agents.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500567","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:21:30Z","timestamp":1744172490000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Design, Synthesis, Characterization and In Vitro Evaluation of Anticholinesterase and Antioxidant Activities of Thiazole\u2013Piperazine Sulphonamide Hybrids"],"prefix":"10.1002","volume":"22","author":[{"given":"Kethineni","family":"Sajitha","sequence":"first","affiliation":[{"name":"Department of Chemistry Sri Venkateswara University  Tirupati Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kandrakonda Yelamanda","family":"Rao","sequence":"additional","affiliation":[{"name":"Bioorganic Chemistry Research Laboratory Department of Chemistry Yogi Vemana University  Kadapa Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valaparla Bala","family":"Yesu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sri Venkateswara University  Tirupati Andhra Pradesh India"},{"name":"Department of Chemistry SGK Government Degree College  Vinukonda Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remya","family":"Chandran","sequence":"additional","affiliation":[{"name":"Laboratory for Computational and Structural Biology Jubilee Centre for Medical Research Jubilee Mission Medical College and Research Institute  Thrissur Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. V.","family":"Dileep","sequence":"additional","affiliation":[{"name":"Laboratory for Computational and Structural Biology Jubilee Centre for Medical Research Jubilee Mission Medical College and Research Institute  Thrissur Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. V. P. 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