{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:30:02Z","timestamp":1790199002079,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T00:00:00Z","timestamp":1751155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K19414"],"award-info":[{"award-number":["22K19414"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic kidney disease (CKD) is a major global health issue, requiring accurate and noninvasive renal function assessment. Glomerular filtration rate (GFR) is the standard measure, but current methods, including inulin clearance and creatinine\u2010based estimation, have limitations in accuracy, invasiveness, and practicality. We investigated\n                    <jats:sc>d<\/jats:sc>\n                    \u2010serine and\n                    <jats:sc>d<\/jats:sc>\n                    \u2010asparagine as novel endogenous biomarkers for GFR evaluation. Their clearance strongly correlates with inulin clearance, demonstrating high reliability and reduced muscle mass dependency compared to creatinine\u2010based methods. Our findings suggest that\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acids offer a promising, less invasive alternative for precise renal function assessment, aiding early CKD detection and management. In addition,\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acids have recently been reported to be useful as biomarkers not only in the renal field but also in various other fields such as malignancy and infectious diseases. This article highlights the potential of\n                    <jats:sc>d<\/jats:sc>\n                    \u2010amino acids in enhancing CKD diagnosis and management.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500579","type":"journal-article","created":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T13:24:33Z","timestamp":1751203473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Theoretical Analysis of\n                    <scp>d<\/scp>\n                    \u2010Serine and\n                    <scp>d<\/scp>\n                    \u2010Asparagine as Biomarkers for Glomerular Filtration Rate"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1144-7820","authenticated-orcid":false,"given":"Ryo","family":"Tanaka","sequence":"first","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Kakuta","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Nonomura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8186-094X","authenticated-orcid":false,"given":"Tomonori","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Nephrology Osaka University Graduate School of Medicine  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010024\u201000820\u20106"},{"issue":"6","key":"e_1_2_11_3_1","first-page":"319","article-title":"Latest Trends in Clinical Practice Guidelines Series: CKD Clinical Practice Guidelines 2023","volume":"82","author":"Abe M.","year":"2023","journal-title":"Commentary Nihon University Journal of Medicine"},{"key":"e_1_2_11_4_1","unstructured":"Japanese Society of Nephrology(Nihon Jinzo Gakkai).Japanese Society for Dialysis Therapy (Nihon Toseki Igakkai); Japan Society for Transplantation (Nihon Ishoku Gakkai); Japanese Society of Clinical Renal Transplantation (Nihon Rinsho Jin Ishoku Gakkai); Japanese Society for Peritoneal Dialysis (Nihon Fukumaku Toseki Igakkai). Strategies and Actual Conditions of Renal Failure Treatment. (2024)."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.14745"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/43.6.913"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.23750\/abm.v91i4.9304"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2008.12.034"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0291620"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26137"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfae279"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300817120"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.06.028"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2017.00082"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157\u2010020\u201001862\u20103"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202403332"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.34067\/KID.0004282021"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157\u2010021\u201002052\u20105"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201041608\u20100"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2023.03.009"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2021.101223"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010024\u201003400\u2010x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2022.166584"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2023.101452"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.150701"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2023.08.002"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.34067\/KID.0000000000000345"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202500018"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500579","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:30:29Z","timestamp":1752075029000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202500579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,29]]},"references-count":27,"alternative-id":["10.1002\/cbdv.202500579"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202500579","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,29]]},"assertion":[{"value":"2025-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00579"}}