{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T17:05:58Z","timestamp":1774026358407,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The gastroprotective effects of the fractions of ethanol extract of <jats:italic>Moringa oleifera<\/jats:italic> leaves (MOEE) were examined. Gastric antisecretory effect and antimicrobial activity against <jats:italic>Helicobacter pylori<\/jats:italic> were used as indices of gastroprotection. MOEE was fractionated to yield five major fractions (K1\u2013K5). Preliminary quantitative phytochemical analysis was carried out on the fractions using standard methods. A total of 40 rats were selected through randomization into eight different groups for the antisecretory activity screening, each group consists of five rats. Groups 1, 2, and 8 served as normal, negative, and positive controls, respectively, whereas Groups 3\u20137 were administered histamine and 200\u00a0mg\/kg of K1\u2013K5, respectively. Gastric acid output was then measured. For the antimicrobial study, the fractions were tested for activity against 10 different strains of <jats:italic>H. pylori<\/jats:italic> using in vitro agar diffusion assay. Results revealed the presence of several bioactive compounds in varying proportions. The fraction K3 had a significant decrease in histamine\u2010induced gastric acid secretion at <jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05. K3 was also the most active against the different strains of <jats:italic>H. pylori<\/jats:italic>. It was concluded that the ethanol extract of <jats:italic>M. oleifera<\/jats:italic> leaves possesses gastroprotective effects as demonstrated by its ability to inhibit gastric acid hypersecretion and <jats:italic>H. pylori<\/jats:italic> activity. It is also safe to say that the active principles of <jats:italic>M. oleifera<\/jats:italic> leaves are present in K3.<\/jats:p>","DOI":"10.1002\/cbdv.202500580","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T19:10:06Z","timestamp":1748977806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fraction K3 From <i>Moringa oleifera<\/i> Exhibits Gastroprotective Effects Through Inhibitory Activity Against <i>Helicobacter pylori<\/i> and Gastric Acid Secretion"],"prefix":"10.1002","author":[{"given":"Mohammed","family":"Alissa","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory, College of Applied Medical Sciences Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kizito Eneye","family":"Bello","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Natural Science Kogi State (Prince Abubakar Audu) University  Anyigba Kogi Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghadah S.","family":"Abusalim","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, College of Applied Medical Sciences Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Helicobacter pylori: Physiology and Genetics","author":"Asaka M.","year":"2001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0B013E328311A65F"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/APHA.12208"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818005.ch3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1254\/JJP.57.495"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.103284"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/MEDICINA59010006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JSPS.2024.102101"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6475"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.26538\/TJNPR\/V7I12.44"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004\u201028032011000400011"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201013\u2010305"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JEP.2005.03.035"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2015.1029050"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10030343"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2023"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.5897\/JMPR2013.5353"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2021.1955921"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.SAJB.2019.07.049"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOPHA.2016.12.107"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.5154\/R.RCHSH.2015.07.018"},{"key":"e_1_2_10_23_1","volume-title":"Medicinal Spices and Vegetables From Africa: Therapeutic Potential Against Metabolic, Inflammatory, Infectious and Systemic Diseases","author":"Kuete V. 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