{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:01:43Z","timestamp":1785484903912,"version":"3.56.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Endophytic fungi are well recognized as prolific producers of structurally diverse natural products. In this study, three endophytic fungal isolates \u2212\n                    <jats:italic>Penicillium singorense<\/jats:italic>\n                    MA\u2010S1, MA\u2010S3, and PDA\u2010S2 \u2013 were obtained in Sasmuan mangrove forest, which had previously been affected and altered by the lahar from the 1991 eruption of Mt. Pinatubo. Morphological characterization and 18S rDNA\/ITS sequencing confirmed their taxonomic placement in the\n                    <jats:italic>Penicillium<\/jats:italic>\n                    genus. The three isolates were subjected to fermentation using rice medium, and metabolites were extracted using ethyl acetate. Crude extracts were then fractionated using the modified Kupchan method, followed by solid\u2010phase extraction (SPE). Disc diffusion assays indicated that the dichloromethane and methanol fractions, as well as the 25% and 100% methanol SPE fractions, exhibited the highest zone of inhibition (ZOI) against the clinical isolates, surpassing the ZOI of voriconazole. Liquid chromatography\u2010tandem mass spectrometry analysis of the fractions, combined with Global Natural Products Social (GNPS) molecular networking, putatively identified the presence of known bioactive metabolites, including diketopiperazines, peptides, and terpenes. Furthermore, other clusters in the network lacked spectral matches in the GNPS library, suggesting the presence of putatively novel metabolites in the extracts. Overall, the study highlights the potential of endophytic fungi from a post\u2010volcanic mangrove ecosystem as valuable sources of bioactive natural products.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500596","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T18:35:33Z","timestamp":1762799733000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Annotation and Bioactivity Profiling of Endophytic Fungi,\n                    <i>Penicillium singorense<\/i>\n                    Species, From the Lahar\u2010altered Sasmuan Mangrove Forest, Pampanga, Philippines by Molecular Networking"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7324-3802","authenticated-orcid":false,"given":"Kevin Jace","family":"Miranda","sequence":"first","affiliation":[{"name":"Department of Physical Sciences and Mathematics, College of Arts and Sciences University of the Philippines Manila Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levi Nicole Gabrielle","family":"Buenafe","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Czarren Kenji Delos","family":"Reyes","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasper Cedrick","family":"Atienza","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaycie Liej","family":"Bernal","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fleurdeliz","family":"Maglangit","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Science, College of Science University of the Philippines Cebu  Cebu City Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Royce Marjheane","family":"Arcigal","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogie Royce","family":"Carandang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Adamson University  Manila Philippines"},{"name":"Department of Health, Behavior, and Society University of Texas at San Antonio School of Public Health San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","unstructured":"A.deBary Morphologie Und Physiologie Der Pilze Flechten Und Myxomyceten(1866) https:\/\/doi.org\/10.5962\/bhl.title.120970.","DOI":"10.5962\/bhl.title.120970"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof10120863"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.791033"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1092105"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/app15031253"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00780"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12040656"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2024.113334"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.ME11207"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63922\u20100"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1002\u20100160(19)60823\u20104"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169\u2010555X(01)00155\u20106"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/00380768.2003.10410047"},{"key":"e_1_2_10_15_1","first-page":"200","article-title":"Comparative Assessment of Natural, Colonized and Planted Mangroves Disturbed by Lahar Deposition in Sasmuan, Pampanga, Philippines","volume":"14","author":"Joson M. 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