{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:42:30Z","timestamp":1773013350205,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Sophora moorcroftiana<\/jats:italic>\n                    , widely distributed in Xizang and rich in alkaloids, warrants further exploration and utilization. This study aims to analyze the efficiency of enzyme\u2010assisted ultrasonic extraction with the standard solvent method comparatively for the qualitative and quantitative analysis of alkaloids in\n                    <jats:italic>S. moorcroftiana<\/jats:italic>\n                    seeds. The research identified optimal extraction conditions, which included an enzyme (cellulose\u2013hemicellulose complex enzyme) concentration of 10% (w\/w), an ultrasonic duration of 45\u00a0min, an ultrasonic power of 267.01\u00a0W, a solid\u2013liquid ratio of 1:44.82 (v\/v), and an ethanol concentration of 77.18%. Under these conditions, the extraction efficiency reached 90.65%, and successfully isolated sophocarpine with a purity of 91.41%. The seeds contained 6.85% moisture and 4.00% alkaloids, with oxymatrine accounting for 2.45%, oxysophocarpine 0.35%, matrine 0.73%, and sophocarpine 0.47%. In addition, the employment of enzymatic hydrolysis\u2010assisted ultrasonic coupling extraction methodology resulted in a 32.5% increase in extraction efficiency compared to the conventional solvent extraction approach. Total alkaloids and the compound sophocarpine demonstrate significant inhibitory effects on both\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    . This study demonstrates that ultrasound\u2010assisted enzyme\u2010coupled extraction is a sustainable and efficient technique with potential applications in the extraction of natural products.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500635","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T18:09:18Z","timestamp":1753898958000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficient Extraction of Alkaloids From\n                    <i>Sophora moorcroftiana<\/i>\n                    Seeds via Enzyme and Ultrasound\u2010Assisted Coupling and Their Antibacterial Activity"],"prefix":"10.1002","volume":"22","author":[{"given":"Sidan","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Chemical Engineering Nanjing Forestry University  Nanjing People's Republic of 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