{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:51:32Z","timestamp":1790387492043,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Phyllanthus niruri<\/jats:italic>\n                    , a well\u2010known medicinal plant, has been widely recognized for its hepatoprotective, anti\u2010inflammatory, and antioxidant properties. In the present study, the hepatoprotective potential of ethanol extract of\n                    <jats:italic>P. niruri<\/jats:italic>\n                    (EPN) and hexane extract of\n                    <jats:italic>P. niruri<\/jats:italic>\n                    (HPN) were evaluated against carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2010induced hepatotoxicity in rats. Oral administration of both EPN and HPN extracts at the dose of 200\u00a0mg\/kg body weight and silymarin (50\u00a0mg\/kg) was assessed for their therapeutic effect on liver function, oxidative stress markers, and inflammatory biomarkers. The results demonstrated that standard drug and\n                    <jats:italic>P. niruri<\/jats:italic>\n                    extracts significantly reduced elevated levels of liver enzymes (ALT and AST), oxidative stress (MDA and NO) markers, and inflammatory markers (TNF\u2010\u03b1 and TGF\u2010\u03b2), along with remarkable restoration of antioxidants (GSH and CAT). Furthermore, histopathological outcomes suggested that the hepatic architecture of liver tissues normalized on extract treatment in comparison to the CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010treated group. The in silico study revealed that 1H\u2010indole, 4\u2010(3\u2010methyl\u20102\u2010butenyl)\u2010 and \u03b1\u2010Amyrin from EPN extract, while phenol, 2,4\u2010bis(1,1\u2010dimethylethyl) and Stigmast\u20104\u2010en\u20103\u2010one from HPN extract exhibited high binding affinities against selected proteins, TNF\u2010\u03b1 and TGF\u2010\u03b2. These findings highlight\n                    <jats:italic>P. niruri<\/jats:italic>\n                    L. as a valuable source for the development of natural hepatoprotective drugs, as evidenced by its ability to mitigate CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced liver toxicity in rats primarily by enhancing antioxidant status and stabilizing liver cell integrity.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500691","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T15:23:46Z","timestamp":1748964226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hepatoprotective Potential of\n                    <i>Phyllanthus niruri<\/i>\n                    Extracts Against CCl\n                    <sub>4<\/sub>\n                    \u2010Induced Liver Injury in Rats: Insight From Phytochemical Profiling, Molecular Docking, and Oxidative Stress Studies"],"prefix":"10.1002","author":[{"given":"Fatima","family":"Arshad","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awais","family":"Altaf","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Raza","family":"Arshad","sequence":"additional","affiliation":[{"name":"Aziz Bhatti Shaheed Teaching Hospital Gujrat Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Sarwar","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahir","family":"Maqbool","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asia","family":"kiran","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Zahid","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology, The University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0905-8076","authenticated-orcid":false,"given":"Tariq","family":"Aziz","sequence":"additional","affiliation":[{"name":"Department of Agriculture Laboratory of Animal Health, Food Hygiene and Quality, University of Ioannina Arta Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khairiah Mubarak","family":"Alwutayd","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science Princess Nourah bint Abdulrahman University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwag","family":"Shami","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science Princess Nourah bint Abdulrahman University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fakhria A.","family":"Al\u2010Joufi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology College of Pharmacy, Jouf University Aljouf Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher S.","family":"Alwethaynani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences College of Applied Medical Sciences, Shaqra University, Alquwayiyah Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/livers2040023"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-014-0145-y"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2016.07.020"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.02.012"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.02.015"},{"issue":"9","key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.7150\/ijms.46108","article-title":"Baicalein Neutralizes Hypercholesterolemia\u2010Induced Aggravation of Oxidative Injury in Rats","volume":"17","author":"AlSaad A. 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