{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:52:46Z","timestamp":1790830366267,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A total of 46 endophytic fungi were isolated from <jats:italic>Huperzia serrata<\/jats:italic> and then cultured in a standard liquid medium and PDB medium. Cytotoxicity and antimicrobial activity assays revealed that seven crude extracts exhibited significant inhibitory effects on MCF\u20107 and SH\u2010SY5Y cell lines; four crude extracts effectively inhibited the growth of <jats:italic>Candida albicans<\/jats:italic>, and 12 crude extracts demonstrated potent inhibition effects on <jats:italic>Staphylococcus aureus<\/jats:italic>. Strains displaying antimicrobial activity or potential for producing novel metabolites were selected for identification. In total, 14 strains representing 7 genera were identified, 5 of which had not been previously documented regarding their secondary metabolites. A fungus, <jats:italic>Penicillium panissanguineum<\/jats:italic> SZSS 4\u20102\u20102 was preliminarily selected for further chemical investigation. From the fermentation extract of <jats:italic>P. panissanguineum<\/jats:italic> SZSS 4\u20102\u20102, one new compound (<jats:bold>1<\/jats:bold>), one new natural product (<jats:bold>2<\/jats:bold>), and two known compounds (<jats:bold>3<\/jats:bold> and <jats:bold>4<\/jats:bold>) were isolated. The structures of these compounds were elucidated through spectral analysis and calculated nuclear magnetic resonance and electronic circular dichroism data. Compound <jats:bold>1<\/jats:bold> demonstrated a significantly stronger inhibitory effect on lipopolysaccharide\u2010induced nitric oxide (NO) production in RAW 264.7 macrophages, with an IC<jats:sub>50<\/jats:sub> value of 64.91 \u00b5M. By contrast, compounds <jats:bold>2<\/jats:bold>, <jats:bold>3<\/jats:bold>, and <jats:bold>4<\/jats:bold> exhibited only moderate inhibitory effects on NO production.<\/jats:p>","DOI":"10.1002\/cbdv.202500701","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T07:54:31Z","timestamp":1742284471000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diversity of Endophytic Fungi Isolated From <i>Huperzia serrata<\/i> and Research on the Secondary Metabolites of <i>Penicillium panissanguineum<\/i> SZSS 4\u20102\u20102"],"prefix":"10.1002","author":[{"given":"Ya\u2010Shuai","family":"Kang","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Wen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Ma","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Yao","family":"Luo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Er\u2010Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Institutional Center for Shared Technologies and Facilities, Institute of Microbiology Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Luo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Zhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8998-1508","authenticated-orcid":false,"given":"Chang\u2010Wei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Beijing Institute of Pharmacology and Toxicology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.2307\/3545919","article-title":"Endophyte: The Evolution of a Term, and Clarification of Its Use and Definition","volume":"73","author":"Wilson D.","year":"1995","journal-title":"Oikos"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1046\/j.1461-0248.2000.00159.x","article-title":"Are Tropical Fungal Endophytes Hyperdiverse?","volume":"3","author":"Arnold A. E.","year":"2000","journal-title":"Ecology Letters"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0071805","article-title":"Isolation, Purification and Characterization of Vinblastine and Vincristine From Endophytic Fungus Fusarium oxysporum Isolated From Catharanthus Roseus","volume":"8","author":"Kumar A.","year":"2013","journal-title":"PLoS ONE"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.fitote.2018.02.031","article-title":"Phenolic Bisabolane Sesquiterpenoids From a Thai Mangrove Endophytic Fungus, Aspergillus Sp. Xy02","volume":"127","author":"Wang P.","year":"2018","journal-title":"Fitoterapia"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1007\/s10600-020-03165-1","article-title":"A New Phenolic Acid Derivative From an Insect\u2010Derived Fungus Nigrospora sphaerica (Strain No. ZMT05)","volume":"56","author":"Ding W. J.","year":"2020","journal-title":"Chemistry of Natural Compounds"},{"key":"e_1_2_9_7_1","first-page":"1862","article-title":"Secondary Metabolites From Colletotrichum fioriniae F18, an Endophytic Fungus Isolated From the Medicinal Plant Mahonia fortunei","volume":"53","author":"Wang H. H.","year":"2018","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.3389\/fmicb.2020.01495","article-title":"Antifungal Nafuredin and Epithiodiketopiperazine Derivatives From the Mangrove\u2010Derived Fungus Trichoderma harzianum D13","volume":"11","author":"Zhao D. L.","year":"2020","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1126\/science.8097061","article-title":"Taxol and Taxane Production by Taxomyces andreanae, an Endophytic Fungus of Pacific Yew","volume":"260","author":"Stierle A.","year":"1993","journal-title":"Science"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.19045\/bspab.2019.80071","article-title":"Study of Antidiabetic Activities of Endophytic Fungi Isolated From Plants","volume":"8","author":"Khan R.","year":"2019","journal-title":"Pure and Applied Biology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.phymed.2013.10.020","article-title":"Piperine Production by Endophytic Fungus Colletotrichum gloeosporioides Isolated From Piper nigrum","volume":"21","author":"Chithra S.","year":"2014","journal-title":"Phytomedicine"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1007\/s00344-010-9139-y","article-title":"Diversity and Antimicrobial and Plant\u2010Growth\u2010Promoting Activities of Endophytic Fungi in Dendrobium loddigesii Rolfe","volume":"29","author":"Chen X. M.","year":"2010","journal-title":"Journal of Plant Growth Regulation"},{"key":"e_1_2_9_13_1","first-page":"3597","article-title":"Ecological Distribution of Arbuscular Mycorrhizal Fungi and the Influencing Factors","volume":"37","author":"Xiang D.","year":"2017","journal-title":"Acta Ecologica Sinica"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"9159","DOI":"10.1007\/s00253-020-10894-4","article-title":"Five Novel and Highly Efficient Endophytic Fungi Isolated From Huperzia serrata Expressing Huperzine A for the Treatment of Alzheimer's Disease","volume":"104","author":"Han W. X.","year":"2020","journal-title":"Applied Microbiology and Biotechnology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1517\/13543784.17.2.209","article-title":"An Update on huperzine A as a Treatment for Alzheimer's Disease","volume":"17","author":"Little J. T.","year":"2008","journal-title":"Expert Opinion on Investigational Drugs"},{"key":"e_1_2_9_16_1","first-page":"399","article-title":"Advances in Studies on Chemical Constituents of Huperzia serrata and Their Pharmacological Effects","volume":"43","author":"Yuan J. Q.","year":"2012","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.phytochem.2013.07.025","article-title":"Ceriponols A\u2013K, Tremulane Sesquiterpenes From Ceriporia Lacerate HS\u2010ZJUT\u2010C13A, a Fungal Endophyte of Huperzia serrata","volume":"95","author":"Ying Y. M.","year":"2013","journal-title":"Phytochemistry"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.fitote.2016.12.012","article-title":"Four New Steroids From the Endophytic Fungus Chaetomium Sp. M453 Derived of Chinese Herbal Medicine Huperzia serrata","volume":"117","author":"Yu F. X.","year":"2017","journal-title":"Fitoterapia"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1080\/14786419.2018.1434648","article-title":"Acetylcholinesterase Inhibitory Active Metabolites From the Endophytic Fungus Colletotrichum Sp. YMF432","volume":"33","author":"Li Z.","year":"2019","journal-title":"Natural Product Research"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.phytol.2016.07.003","article-title":"Penicisimpins A\u2010C, Three New Dihydroisocoumarins From Penicillium Simplicissimum MA\u2010332, a Marine Fungus Derived From the Rhizosphere of the Mangrove Plant Bruguiera sexangula Var. Rhynchopetala","volume":"17","author":"Xu R.","year":"2016","journal-title":"Phytochemistry Letters"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1039\/j39660001608","article-title":"The Structure of Pulvilloric Acid","author":"McOmie J. F. W.","year":"1966","journal-title":"Journal of the Chemical Society [Section] C: Organic"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fitote.2020.104530","article-title":"Chemical Constituents From Wetland Soil Fungus Penicillium oxalicum GY1","volume":"142","author":"Ding T.","year":"2020","journal-title":"Fitoterapia"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/jlac.199719970131","article-title":"Enantioselective Synthesis of ( R )\u2010(+)\u2010Pulvilloric Acid","volume":"28","author":"Roedel T.","year":"1997","journal-title":"Liebigs Annalen\/Recueil"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1080\/10286020.2018.1495197","article-title":"Three New \u03b1\u2010pyrone Derivatives From the Plant Endophytic Fungus Penicillium ochrochloronthe and Their Antibacterial, Antifungal, and Cytotoxic Activities","volume":"21","author":"Zhao T.","year":"2019","journal-title":"Journal of Asian Natural Products Research"},{"key":"e_1_2_9_25_1","first-page":"2133","article-title":"Isolation and Identification of Huperzine A\u2010producing Endophytic Fungus SNZ\u201012 From Huperzia serrata","volume":"30","author":"Zhang H. Q.","year":"2018","journal-title":"Natural Products Research and Development"},{"key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.3390\/md12041815","article-title":"Seven New and Two Known Lipopeptides as Well as Five Known Polyketides: The Activated Production of Silent Metabolites in a Marine\u2010Derived Fungus by Chemical Mutagenesis Strategy Using Diethyl Sulphate","volume":"12","author":"Wu C. J.","year":"2014","journal-title":"Marine Drugs"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/S0023-6438(03)00088-4","article-title":"Antimicrobial Activity of Essential Oils on the Native Microflora of Organic Swiss Chard","volume":"36","author":"Ponce A. G.","year":"2003","journal-title":"Lebensmittel\u2010Wissenschaft und\u2010Technologie"},{"key":"e_1_2_9_28_1","volume-title":"Performance Standards for Antifungal Susceptibility Testing of Yeasts; Document M60 Standard\u2010Second Edition","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2020"},{"key":"e_1_2_9_29_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing, Document M100 Standard\u2010Thirty\u2010two Edition","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2022"},{"key":"e_1_2_9_30_1","doi-asserted-by":"crossref","DOI":"10.1002\/cbdv.202401921","article-title":"Pestiorosins A\u2013F, New Papulacandins Isolated from the Fungus Pestalotiopsis Rosea YNJ21","volume":"22","author":"Lu Y.","year":"2024","journal-title":"Chemistry & Biodiversity"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2020.113698","article-title":"Ethyl Acetate Fraction From Nymphaea Hybrida Peck Modulates Inflammatory Responses in LPS\u2010stimulated RAW 264.7\u00a0Cells and Acute Inflammation Murine Models","volume":"269","author":"Zhang Z. W.","year":"2020","journal-title":"Journal of Ethnopharmacolgy"},{"key":"e_1_2_9_32_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioorg.2021.105111","article-title":"Furanaspermeroterpenes A and B, Two Unusual Meroterpenoids With a Unique 6\/6\/6\/5\/5 Pentacyclic Skeleton from the Marine\u2010Derived Fungus Aspergillus terreus GZU\u201031\u20101","volume":"114","author":"Tang Y. Q.","year":"2021","journal-title":"Bioorganic Chemistry"},{"key":"e_1_2_9_33_1","unstructured":"Gaussian Gaussian 16 Revision A.03(Gaussian 2016)."}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T06:23:53Z","timestamp":1743834233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202500701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":32,"alternative-id":["10.1002\/cbdv.202500701"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202500701","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]},"assertion":[{"value":"2025-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202500701"}}