{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:42Z","timestamp":1786977342344,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic myeloid leukemia (CML) is a malignant hematological neoplasm. FA\u20102\u2010b\u2010\u03b2, extracted from\n                    <jats:italic>Agaricus blazei<\/jats:italic>\n                    Murrill, has demonstrated antitumor activity against multiple cancer types. Nevertheless, the specific pharmacodynamic components and molecular mechanisms of FA\u20102\u2010b\u2010\u03b2 against CML remain to be elucidated. This study aimed to systematically investigate the mechanism of FA\u20102\u2010b\u2010\u03b2 against CML through an integrated strategy combining liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS), network pharmacology prediction, and in vitro experimental validation. Using LC\u2013MS\/MS profiling, we identified a total of 126 chemical components. Subsequently, network pharmacological analysis screened 45 potentially active components and 129 CML\u2010related overlapping targets. Further pathway enrichment analysis revealed that these candidate targets were predominantly involved in the PI3K\/AKT and MAPK pathways. In vitro validation confirmed the suppression of PI3K\/AKT phosphorylation in K562 cells. Collectively, this study provides significant insights into the active components and antitumor mechanisms of FA\u20102\u2010b\u2010\u03b2, emphasizing its potential as a promising therapeutic agent for treating CML.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500758","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T18:34:26Z","timestamp":1753554866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Network Pharmacology and In Vitro Experiments Reveal the Mechanism of\n                    <i>Agaricus blazei<\/i>\n                    Murill Extract in Treating Chronic Myeloid Leukemia"],"prefix":"10.1002","volume":"22","author":[{"given":"Dongping","family":"Wang","sequence":"first","affiliation":[{"name":"Gansu University of Chinese Medicine  Lanzhou China"},{"name":"Gansu Provincial Hospital  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Cuiying Biomedical Research Center Lanzhou University Second Hospital  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanwen","family":"Ge","sequence":"additional","affiliation":[{"name":"Cuiying Biomedical Research Center Lanzhou University Second Hospital  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9643-5284","authenticated-orcid":false,"given":"Yanqing","family":"Sun","sequence":"additional","affiliation":[{"name":"Gansu University of Chinese Medicine  Lanzhou China"},{"name":"Gansu Provincial Hospital  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)01204-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/243290a0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.27443"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-023-00823-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27238367"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7090728"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612823666170119093719"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2022.10.029"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i31.4185"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2007001100015"},{"key":"e_1_2_10_12_1","first-page":"2889","article-title":"Studies on the Host\u2010Mediated Antitumor Polysaccharides.13. Antitumor\u2010Activity and Some Properties of Water\u2010Soluble Polysaccharides From Himematsutake, the Fruiting Body of Agaricus blazei Murill","volume":"54","author":"Mizuno T.","year":"1990","journal-title":"Agricultural and Biological Chemistry"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41540-020-00168-0"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113817"},{"key":"e_1_2_10_15_1","first-page":"1560","article-title":"Agaricus blazei Murill Extract FA\u20102\u2010b\u2010\u03b2 Inhibits Gastric Cancer Cell Proliferation Through the Mechanisms of Cell\u2010Cycle Blocking and Apoptosis by the Mitochondrial Pathway","volume":"47","author":"Junshun F.","year":"2023","journal-title":"Asian Journal of Surgery"},{"key":"e_1_2_10_16_1","first-page":"1631","article-title":"Correlation of the Apoptosis in the Mitochondrial Pathway With the JAK2\/STAT3 Signaling Pathway Regarding the Effect of Agaricus blazei Murill Extract FA\u20102\u2010b\u2010\u03b2 in Inhibiting the Proliferation of Gastric Cancer Cells","volume":"47","author":"Junshun F.","year":"2023","journal-title":"Asian Journal of Surgery"},{"key":"e_1_2_10_17_1","first-page":"1069","article-title":"Apoptotic Effect of Selenium Mushroom Extract From Qinba on Multiple Myeloma Cells","volume":"38","author":"Yang G.","year":"2022","journal-title":"Histology and Histopathology"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116077"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16020205"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.5387"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2022.26.3.145"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13184677"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8727935"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204281"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01827-6"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2020.66.6.15"},{"key":"e_1_2_10_27_1","first-page":"503","article-title":"Tanshinone IIA Enhances the Inhibitory Effect of Imatinib on Proliferation and Motility of Acute Leukemia Cell Line TIB\u2011152 In Vivo and In Vitro by Inhibiting the PI3K\/AKT\/mTOR Signaling Pathway","volume":"43","author":"Teng Z.","year":"2020","journal-title":"Oncology Reports"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.23817"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000490142"},{"key":"e_1_2_10_30_1","first-page":"626","article-title":"PTEN Is a Tumor Suppressor in CML Stem Cells and BCR\u2010ABL\u2010Induced Leukemias in Mice","volume":"115","author":"Cong P.","year":"2009","journal-title":"Blood"},{"key":"e_1_2_10_31_1","first-page":"4285","article-title":"Preliminary Results Indicate Resveratrol Affects Proliferation and Apoptosis of Leukemia Cells by Regulating PTEN\/PI3K\/AKT Pathway","volume":"23","author":"Meng J.","year":"2019","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz382"},{"key":"e_1_2_10_33_1","first-page":"D845","article-title":"The DisGeNET Knowledge Platform for Disease Genomics: 2019 Update","volume":"48","author":"Janet P.","year":"2019","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_10_34_1","first-page":"D638","article-title":"The STRING Database in 2023: Protein\u2013Protein Association Networks and Functional Enrichment Analyses for Any Sequenced Genome of Interest","volume":"51","author":"Damian S.","year":"2022","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac194"}],"container-title":["Chemistry &amp; 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