{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:07:37Z","timestamp":1756426057696,"version":"3.44.0"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, the biological activities of water and ethanol extracts from <jats:italic>Sinapis alba<\/jats:italic> and <jats:italic>Brassica nigra<\/jats:italic> seeds were evaluated using various bioanalytical methods and spectrophotometric analyses. Antioxidant capacities were assessed through cupric reducing antioxidant capacity, ferric reducing antioxidant power assay, and ferric ion reducing assays, while radical scavenging abilities were evaluated using 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH)\u2022 and 2,2\u2019\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulphonate) diammonium salt (ABTS)\u2022+ assays. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified as gallic acid equivalents (GAE) and quercetin equivalents (QE), respectively. Additionally, anticholinergic activity was determined by Ellman's method. Qualitative and quantitative analyses of phenolic compounds in extracts were performed using liquid chromatography\u2010tandem mass spectrometry. Reference standards were employed for the comparison. The half\u2010maximal inhibitory concentration (IC<jats:sub>50<\/jats:sub>) values of <jats:italic>S. alba<\/jats:italic> and <jats:italic>B. nigra<\/jats:italic> seed extracts were in the range 1199\u20131799\u00a0\u00b5g\/mL for DPPH\u2022 and 786.6\u20131259\u00a0\u00b5g\/mL for ABTS\u2022+ radical scavenging activities. TPC levels for <jats:italic>S. alba<\/jats:italic> and <jats:italic>B. nigra<\/jats:italic> water and ethanol extracts varied between 12.35 and 28.82\u00a0mg GAE\/g extract, while TFC values ranged from 12.18 to 28.36\u00a0mg QE\/g extract. The extracts also showed potent acetylcholinesterase inhibitory effects, with IC<jats:sub>50<\/jats:sub> values in the range of 0.167\u20130.194\u00a0\u00b5g\/mL. These significant biological activities are attributed to the rich phytochemical composition of the seed extracts, indicating their potential as natural sources of antioxidant and anticholinergic agents.<\/jats:p>","DOI":"10.1002\/cbdv.202500762","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T20:14:35Z","timestamp":1756412075000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Investigation on Yellow (<i>Sinapis alba<\/i>) and Black (<i>Brassica nigra<\/i>) Mustard Seed Extracts: Antioxidant, Anticholinergic Activities, and Phenolic Analysis via Liquid Chromatography\u2010Tandem Mass Spectrometry"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5759-7889","authenticated-orcid":false,"given":"Leyla","family":"Polat K\u00f6se","sequence":"first","affiliation":[{"name":"Department of Pharmacy Services, Vocational School \u0130stanbul Beykent University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Biology and Chemistry of the Cruciferae","author":"Vaughan J. 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