{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:56:24Z","timestamp":1787777784210,"version":"build-2784847793"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>5\u2010Fluorouracil (5\u2010FU), one of the most widely used chemotherapy drugs, is known to cause widespread toxicity and irreparable tissue damage. The purpose of this work was to analyze the phytochemical components of <jats:italic>Prunus laurocerasus<\/jats:italic> (PL) fruit extract using liquid chromatography with tandem mass spectrometry (LC\u2010MS\/MS) and to look into the effectiveness of PL pretreatment on 5\u2010FU\u2010induced liver damage. The study consisted of control (C), PL, 5\u2010FU, PL+5\u2010FU groups, and PL groups received 500\u00a0mg\/kg PL as pretreatment for seven days. On the eighth day, 100\u00a0mg\/kg 5\u2010FU was administered intraperitoneally as a single dose to the PL+5\u2010FU and 5\u2010FU groups. In the LC\u2010MS\/MS results, it was determined that the phenolic compounds found in the foreground in PL were quinic acid and chlorogenic acid. PL pretreatment increased total antioxidant status levels and B\u2010cell lymphoma 2 (Bcl\u20102) expression and also decreased Keap1, total oxidant status, oxidative stress index levels, and Bcl\u20102\u2010associated X protein, Caspase\u20103, and light chain\u20103B expressions. 8\u2010hydroxy\u20102'\u2010deoxyguanosine levels were higher in the 5\u2010FU group compared to the C and PL groups. Moreover, in the PL+5\u2010FU group, the levels were similar to the C, PL, and 5\u2010FU groups. In conclusion, PL pretreatment demonstrated hepatoprotective action via regulating the Kelch\u2010like ECH\u2010associated protein 1\/nuclear factor\u2010erythroid 2\u2010related factor 2 pathway in 5\u2010FU\u2010induced liver damage, lowering oxidative stress, and decreasing apoptosis and autophagy from programmed cell death.<\/jats:p>","DOI":"10.1002\/cbdv.202500798","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T19:11:17Z","timestamp":1748632277000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hepatoprotective Activity of Prunus laurocerasus Fruit Extract Against 5\u2010FU\u2010Induced Liver Injury and LC\u2010MS\/MS Profiling"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2328-9164","authenticated-orcid":false,"given":"G\u00f6zde","family":"Atila Uslu","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3974-5814","authenticated-orcid":false,"given":"Hamit","family":"Uslu","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1711-5499","authenticated-orcid":false,"given":"Taha Abdulkadir","family":"\u00c7oban","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9765-4569","authenticated-orcid":false,"given":"Mustafa","family":"\u00d6zkaraca","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Veterinary Medicine Cumhuriyet University  Sivas T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2722-3290","authenticated-orcid":false,"given":"Ali Sefa","family":"Mendil","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Veterinary Medicine Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5723-2959","authenticated-orcid":false,"given":"Hasan","family":"Can","sequence":"additional","affiliation":[{"name":"East Anatolia High Technology Application and Research Center Atat\u00fcrk University  Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijp.2013.373.378"},{"key":"e_1_2_8_3_1","first-page":"576","article-title":"Micropropagation of Cherry Laurel Prunus laurocerasus L.","volume":"11","author":"Sulusluoglu M.","year":"2013","journal-title":"Journal of Food, Agriculture and Environment"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0344486"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(03)00282-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(98)00133-0"},{"key":"e_1_2_8_7_1","first-page":"430","article-title":"Effects of Different Doses of Prunus laurocerasus L. 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