{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:03:37Z","timestamp":1773493417279,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005672","name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","doi-asserted-by":"publisher","award":["184\/2023"],"award-info":[{"award-number":["184\/2023"]}],"id":[{"id":"10.13039\/501100005672","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mezilaurus duckei<\/jats:italic>\n                    , a Brazilian endemic tree species found exclusively in the Amazon Rainforest, is primarily exploited for timber in construction. Due to its endangered status, this study aimed to investigate the chemical profile and biological properties of the ethanolic extract and its phases derived from\n                    <jats:italic>M. duckei<\/jats:italic>\n                    leaves. Chemical profiling using high\u2010performance liquid chromatography\u2010tandem mass spectrometry revealed flavonoids, including glycosylated kaempferol and its derivatives, as well as \u03b3\u2010butyrolactones, as the major constituents. Additional chromatographic separations led to the isolation of kaempferol, reported for the first time in the\n                    <jats:italic>Mezilaurus<\/jats:italic>\n                    genus, and rubrenolide, identified for the first time in\n                    <jats:italic>M. duckei<\/jats:italic>\n                    . Antioxidant activity, assessed using the 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl method, demonstrated significant free radical scavenging properties of the ethanolic extract (half\u2010maximal inhibitory concentration [IC\n                    <jats:sub>50<\/jats:sub>\n                    ] = 1.43\u00a0\u00b5g\/mL) and the ethyl acetate phase (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.28\u00a0\u00b5g\/mL). Antimicrobial activity, evaluated via the microdilution method, revealed promising activity of the dichloromethane fraction against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    (minimum inhibitory concentration values of 185.5 and 375 \u00b5g\/mL, respectively). Toxicity assessment using the\n                    <jats:italic>Artemia salina<\/jats:italic>\n                    lethality assay confirmed the non\u2010toxic nature of the ethanolic extract (50% lethal dose &gt; 1000\u00a0\u00b5g\/mL). These results highlight the potential of\n                    <jats:italic>M. duckei<\/jats:italic>\n                    as a source of bioactive compounds, undescoring their relevance for further applications.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500826","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T20:00:22Z","timestamp":1757448022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Composition, Antioxidant, and Antibacterial Activities of\n                    <i>Mezilaurus duckei<\/i>\n                    (Lauraceae)"],"prefix":"10.1002","volume":"22","author":[{"given":"Bruna S.","family":"Moroto","sequence":"first","affiliation":[{"name":"Instituto De Bioci\u00eancias Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Machate","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talita V.","family":"Freire","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9rica L.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio M.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto De Bioci\u00eancias Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana C.","family":"Micheletti","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8850-9300","authenticated-orcid":false,"given":"N\u00eddia C.","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Instituto De Qu\u00edmica Universidade Federal de Mato Grosso Do Sul  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pld.2025.04.001"},{"key":"e_1_2_9_3_1","unstructured":"Lauraceae in Flora e Funga do Brasil.Jardim Bot\u00e2nico do Rio de Janeiro. Available at https:\/\/floradobrasil.jbrj.gov.br\/FB84433. Accessed March 1 2025."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010021\u201009791\u2010z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015493\u20105"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8060150"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12229\u2010021\u201009265\u2010z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2023.2260474"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12030452"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127773"},{"key":"e_1_2_9_11_1","volume-title":"Compendium of Crop Genome Designing for Nutraceuticals","author":"Talavera A.","year":"2023"},{"key":"e_1_2_9_12_1","first-page":"195","article-title":"Import\u00e2ncia Econ\u00f4mica Da Fam\u00edlia Lauraceae Linal","volume":"8","author":"Marques C. A.","year":"2001","journal-title":"FLORAM"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2021.105065"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.33448\/rsd\u2010v10i11.19390"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10091854"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10060869"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.15560\/8.6.1323"},{"key":"e_1_2_9_18_1","volume-title":"\u00c1rvores brasileiras: Manual de Identifica\u00e7\u00e3o e Cultivo de Plantas Arb\u00f3reas do Brasil","author":"Lorenzi H.","year":"2002"},{"key":"e_1_2_9_19_1","first-page":"154","volume-title":"Goi\u00e2nia: PRPPGFG","author":"Moraes P. L. R.","year":"2007"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/2175\u20107860201869218"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3464237"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1234488"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0044-59672012000400012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100035\u20101568355"},{"key":"e_1_2_9_25_1","first-page":"469","article-title":"Essential Oil Composition of Dicypellium manausense, Mezilaurus duckei, Mezilaurus itauba and Pleurothyrium vasquezii From Species of the Lauraceae family in the Amazon","volume":"12","author":"Alcantara J. M.","year":"2013","journal-title":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas"},{"key":"e_1_2_9_26_1","unstructured":"Minist\u00e9rio do Meio Ambiente (MMA) Portaria n\u00b0 148 de 7 de junho de 2022 Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade (ICMBio) https:\/\/www.icmbio.gov.br\/cepsul\/images\/stories\/legislacao\/Portaria\/2020\/P_mma_148_2022_altera_anexos_P_mma_443_444_445_2014_atualiza_especies_ameacadas_extincao.pdf Accessed April 10 2025."},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2017.05.023"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00672-0"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.01.002"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.05.124"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112525"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1050907"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations6010010"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11192623"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315054"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2024.112389"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12081642"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1980762"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0245882"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2004.04.037"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37577-5"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100030\u20101250027"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2010.00123"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000001074"},{"key":"e_1_2_9_45_1","unstructured":"J.OliveiraandW. C.Reygaert \u201cGram\u2010Negative Bacteria. [Updated 2023 Aug 8] \u201d inStatPearls [Internet](StatPearls Publishing 2024) https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK538213\/."},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25061340"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25225243"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10121852"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29051000"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010018\u20109591\u2010z"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201074957\u20106"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25184149"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.106166"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-971236"},{"key":"e_1_2_9_55_1","unstructured":"CLSI W. P \u201cReference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts Approved Standard\u2014Third Edition. CLSI Document M27\u2010A3 \u201d (Clinical and Laboratory Standard Institute 2008)."},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46442-0"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.62.1201"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2025.e42518"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2025.117409"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbp.2010.04.008"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2019.04.025"},{"key":"e_1_2_9_62_1","first-page":"13","article-title":"Tres Bioensayos Simples para Quimicos de Productos Naturales","volume":"18","author":"McLaughlin J. L.","year":"1995","journal-title":"Revista de la Sociedad Venezolana de Qu\u00edmica"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500826","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202500826","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202500826","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T11:44:31Z","timestamp":1773488671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202500826"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,9]]},"references-count":61,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/cbdv.202500826"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202500826","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,9]]},"assertion":[{"value":"2025-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00826"}}