{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:42:22Z","timestamp":1773013342678,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Malaria is a significant concern due to the emergence of artemisinin\u2010resistant parasites. This study aims to unveil the selective inhibitory potential of extracts and derivatives of some Niger's plants against\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    . Plant extracts were screened on chloroquine\u2010sensitive (\n                    <jats:italic>Pf<\/jats:italic>\n                    3D7) and multidrug\u2010resistant (\n                    <jats:italic>Pf<\/jats:italic>\n                    Dd2)\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    strains using the SYBR Green method, followed by bio\u2010guided fractionation of active extracts. Bio\u2010guided method was used for the isolation. Resazurin\u2010based and hemoglobin quantification assays were used to assess the selectivity on normal RAW cells and erythrocytes, respectively. Among the 27 extracts, five exhibited pronounced activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0&lt;\u00a05\u00a0\u00b5g\/mL) against multidrug\u2010resistant (\n                    <jats:italic>Pf<\/jats:italic>\n                    Dd2) and three against sensitive (\n                    <jats:italic>Pf<\/jats:italic>\n                    3D7) strains of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    . The methanolic extract from\n                    <jats:italic>Phyllanthus pentandrus<\/jats:italic>\n                    displayed the most promising activity the most active on both strains (IC\n                    <jats:sub>50<\/jats:sub>\n                    <jats:italic>Pf<\/jats:italic>\n                    Dd2\u00a0=\u00a01.85\u00a0\u00b5g\/mL and IC\n                    <jats:sub>50<\/jats:sub>\n                    <jats:italic>Pf<\/jats:italic>\n                    3D7\u00a0=\u00a04.25\u00a0\u00b5g\/mL). Its fractionation led to five fractions, among which the ethyl acetate fraction was the most active (IC\n                    <jats:sub>50<\/jats:sub>\n                    <jats:italic>Pf<\/jats:italic>\n                    Dd2\u00a0=\u00a03.10\u00a0\u00b5g\/mL). Four compounds were isolated from the ethyl acetate fraction: kaempferol, quercetin, gallic acid, and quercetin\u20103\u2010O\u2010rutinoside. Active extracts and fractions showed good selectivity on normal RAW cells and erythrocytes. This study validates the medicinal use of Niger ethnomedicine for the treatment of malaria.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500879","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T19:18:24Z","timestamp":1752866304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bio\u2010guided investigation of nine Niger\u2019s ethnomedicinal plants reveals nature\u2010derived antimalarial chemical pharmacophores for drug discovery"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8785-1292","authenticated-orcid":false,"given":"Mahamane","family":"Idi Issa Abdoulahi","sequence":"first","affiliation":[{"name":"Laboratory of Natural Substances and Organic Synthesis, FAST Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariscal Brice Tchatat","family":"Tali","sequence":"additional","affiliation":[{"name":"Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"},{"name":"Advanced Research and Health Innovation Hub  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilagouma Amadou","family":"Tidjani","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Substances and Organic Synthesis, FAST Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hama Hamadou","family":"Habibou","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Substances and Organic Synthesis, FAST Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul Etoga","family":"Mbarga","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Applied Organic Synthesis University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darline","family":"Dize","sequence":"additional","affiliation":[{"name":"Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauve Rachel Tchokouaha","family":"Yamthe","sequence":"additional","affiliation":[{"name":"Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"},{"name":"Pharmacology Laboratory and Drug Discovery Institute of Medical Research and Medicinal Plant Studies  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael Hermann Kengne","family":"Kamdem","sequence":"additional","affiliation":[{"name":"Drug Discovery and Smart Molecules Research Laboratory, Department of Chemical Sciences University of Johannesburg  Johannesburg South Africa"},{"name":"Centre for Natural Product Research (CNPR), Department of Chemical Sciences University of Johannesburg  Johannesburg South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek Tantoh","family":"Ndinteh","sequence":"additional","affiliation":[{"name":"Drug Discovery and Smart Molecules Research Laboratory, Department of Chemical Sciences University of Johannesburg  Johannesburg South Africa"},{"name":"Centre for Natural Product Research (CNPR), Department of Chemical Sciences University of Johannesburg  Johannesburg South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Mkounga","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Applied Organic Synthesis University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrice Fekam","family":"Boyom","sequence":"additional","affiliation":[{"name":"Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"},{"name":"Advanced Research and Health Innovation Hub  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bakasso","family":"Sahabi","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Substances and Organic Synthesis, FAST Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"World Health Organization World Malaria Report 2022 (WHO 2022) https:\/\/www.who.int\/teams\/global\u2010malaria\u2010programme."},{"key":"e_1_2_11_3_1","unstructured":"World Health Organization World 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