{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T01:29:25Z","timestamp":1788744565424,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Hedysarum<\/jats:italic>\n                    species play a crucial agronomic role due to their high feed quality and ability to enhance soil fertility through nitrogen fixation. Traditionally, they have been used in medicine to boost energy, support the immune system, and benefit the peripheral nervous system. However, the biological activity and phytochemical composition of\n                    <jats:italic>Hedysarum nitidum<\/jats:italic>\n                    remain unexplored. This study aimed to evaluate the bioactivity of methanol (HNM) and water (HNW) extracts from the aerial parts of\n                    <jats:italic>H. nitidum<\/jats:italic>\n                    . Antioxidant (total polyphenol and flavonoid contents, 2,2\u2010diphenyl\u20102\u2010picrylhydrazyl hydrate [DPPH], ferric\u2010reducing antioxidant power [FRAP], Cu (II) ion reducing antioxidant capacity [CUPRAC]), antimicrobial, cytotoxic, and enzyme inhibitory (tyrosinase, cholinesterase, \u03b1\u2010glucosidase, \u03b1\u2010amylase) activities were assessed. Phytochemical and essential oil compositions were analyzed using high\u2010performance liquid chromatography (HPLC) and gas chromatography\u2013mass spectroscophy (GC\u2013MS). HPLC identified\n                    <jats:italic>p<\/jats:italic>\n                    \u2010hydroxybenzoic acid, caffeic acid, and 2\u2010hydroxycinnamic acid, whereas GC\u2013MS detected\n                    <jats:italic>n<\/jats:italic>\n                    \u2010octanal, hexahydrofarnesyl acetone, humulene epoxide\u2010II, \u03b1\u2010terpineol, and eicosane. HNW and HNM exhibited strong antioxidant activity and enzyme inhibition, suggesting potential applications in hyperpigmentation, diabetes, and Alzheimer's treatment. HNM showed selective antibacterial activity against Gram\u2010positive bacteria, whereas neither extract exhibited cytotoxicity against A549 cells. These findings highlight\n                    <jats:italic>H. nitidum<\/jats:italic>\n                    as a promising candidate for therapeutic applications.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202500992","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T10:53:31Z","timestamp":1749812011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comprehensive Analysis of\n                    <i>Hedysarum nitidum<\/i>\n                    : Identification Phytochemical Contents, Antioxidant Potential, Antimicrobial Properties, Cytotoxic Effects, and Enzyme Inhibition Activities"],"prefix":"10.1002","author":[{"given":"Nuray","family":"Balt\u00fcrk","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-5616","authenticated-orcid":false,"given":"Merve","family":"Badem","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyda","family":"Kanbolat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sercan","family":"Y\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011fba Mazlum","family":"\u015een","sequence":"additional","affiliation":[{"name":"Department of Medicinal Biochemistry, Faculty of Medicine Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6zde","family":"Bozdal","sequence":"additional","affiliation":[{"name":"Department of Professional Pharmaceutical Sciences and Botany, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can \u00d6zg\u00fcr","family":"Yal\u00e7\u0131n","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Toxicology, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Kandemir","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Erzincan University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuriye","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sengul Alpay","family":"Karaoglu","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Arts and Sciences Recep Tayyip Erdogan University  Rize T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rezzan","family":"Aliyazicioglu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.2042-7158.2011.01383.x","article-title":"Ethnobotanical and Scientific Aspects of Malva sylvestris L.: A Millennial Herbal Medicine","volume":"2","author":"Gasparetto J. 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