{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:57:28Z","timestamp":1785488248675,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To discover novel laccase inhibitors as potential fungicides, three series of myrtenal hydrazide, carboxamide, and acylthiourea derivatives were designed, synthesized, and assayed for their antifungal property. The majority of these target compounds demonstrated significant in vitro antifungal activity toward\n                    <jats:italic>Monilinia fructicola<\/jats:italic>\n                    ,\n                    <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>\n                    ,\n                    <jats:italic>Valsa mali<\/jats:italic>\n                    , and\n                    <jats:italic>Botryosphaeria dothidea<\/jats:italic>\n                    . Notably, compounds\n                    <jats:bold>3d<\/jats:bold>\n                    and\n                    <jats:bold>3l<\/jats:bold>\n                    exhibited potent inhibition to\n                    <jats:italic>M. fructicola<\/jats:italic>\n                    with median effective concentration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ) values of 0.307 and 0.465\u00a0mg\/L, respectively, which were comparable to carbendazim (EC\n                    <jats:sub>50<\/jats:sub>\n                    = 0.199\u00a0mg\/L). In vivo experiments conducted on pear fruits indicated that\n                    <jats:bold>3d<\/jats:bold>\n                    possessed exceptional protective and curative effects. The morphological study through scanning electron microscopy observation showed that\n                    <jats:bold>3d<\/jats:bold>\n                    caused substantial damage to the mycelial structure of\n                    <jats:italic>M. fructicola<\/jats:italic>\n                    . Additionally, during in vitro laccase inhibition assays, compound\n                    <jats:bold>3d<\/jats:bold>\n                    displayed remarkable inhibition toward laccase, achieving a half\u2010maximal inhibitory concentration value of 4.86 \u00b5M, significantly stronger than the positive control cysteine. These findings suggest that the target myrtenal derivatives hold great promise as lead compounds for the development of laccase\u2010targeting fungicides.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501024","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:55:27Z","timestamp":1761702927000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis and Antifungal Activity of Novel Myrtenal Hydrazide, Carboxamide, and Acylthiourea Derivatives as Potential Laccase Inhibitors\n                    <sup>\u2020<\/sup>"],"prefix":"10.1002","volume":"23","author":[{"given":"Ning","family":"Ou","sequence":"first","affiliation":[{"name":"Jiangsu Co\u2010Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro\u2010forest Biomass, Jiangsu Key Lab of Biomass\u2010Based Green Fuels and Chemicals, College of Chemical Engineering Nanjing Forestry University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ming","family":"Zheng","sequence":"additional","affiliation":[{"name":"Jiangsu Co\u2010Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro\u2010forest Biomass, Jiangsu Key Lab of Biomass\u2010Based Green Fuels and Chemicals, College of Chemical Engineering Nanjing Forestry University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010Nan","family":"Cui","sequence":"additional","affiliation":[{"name":"Jiangsu Co\u2010Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro\u2010forest Biomass, Jiangsu Key Lab of Biomass\u2010Based Green Fuels and Chemicals, College of Chemical Engineering Nanjing Forestry 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