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Griseb. through in vitro, in silico, and phytochemical analyses. The cytotoxic activity of\n                    <jats:italic>O. hispidus<\/jats:italic>\n                    was evaluated against four different cancer cell lines \u2013 lung (A549), breast (MCF\u20107), liver (HepG2), and cervix (HeLa) \u2013 as well as one healthy cell line (Beas\u20102B), using the 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide colorimetric assay.\n                    <jats:italic>n\u2010<\/jats:italic>\n                    Hexane and dichloromethane sub\u2010extracts with the IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 9.77 \u00b1 0.57 and 7.10 \u00b1 0.78\u00a0mg\/mL on A549, respectively, were fractionated through chromatographic techniques, and the structures of the isolated compounds were elucidated using various spectroscopic methods. A novel compound, 2',6'\u2010dihydroxy\u20104'\u2010methoxy\u20103''\u2010(\u0264,\u0264\u2010dimethylallyl)\u20104''\u2010hydroxy\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010hydroxydihydrochalcone (\n                    <jats:bold>3<\/jats:bold>\n                    ), along with three known coumarins \u2013 umbelliferone 6\u2010carboxylic acid (\n                    <jats:bold>1<\/jats:bold>\n                    ), umbelliferone (\n                    <jats:bold>2<\/jats:bold>\n                    ), and nodakenetin (\n                    <jats:bold>4<\/jats:bold>\n                    ) \u2013 were isolated from the active fractions. Compound\n                    <jats:bold>3<\/jats:bold>\n                    , which possesses a chalcone structure, exhibited notable cytotoxicity against lung cancer cells with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 13.72 \u00b1 0.80\u00a0\u00b5M.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501077","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T18:10:08Z","timestamp":1753207808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cytotoxic Activity of\n                    <i>Opopanax hispidus<\/i>\n                    (Friv.) Griseb.: Characterization of a New Chalcone\u2010type Compound"],"prefix":"10.1002","volume":"22","author":[{"given":"Kevser","family":"Taban","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Sivas Cumhuriyet University Sivas T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4201-1325","authenticated-orcid":false,"given":"\u0130pek","family":"S\u00fcntar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Gazi University Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perihan","family":"G\u00fcrb\u00fcz","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra","family":"Emerce","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Toxicology, Faculty of Pharmacy Gazi University Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osman","family":"Tugay","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy Selcuk University Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015eeng\u00fcl Dilem","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Pharmacy Erciyes University, Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beyza","family":"Hamur","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Basic Sciences Gebze Technical University Gebze, Kocaeli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet Ceyhan","family":"G\u00f6ren","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Basic Sciences Gebze Technical University Gebze, Kocaeli T\u00fcrkiye"},{"name":"Troyasil HPLC Column Technologies Doruk Analitik, Mehmet Akif Mah. 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