{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:09:02Z","timestamp":1775070542942,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Huayu Jiedu formula (HYJDF), a traditional Chinese herbal compound effective against acute cerebral infarction (ACI), lacks a defined mechanism of action. HYJDF's bioactive components were identified via ultra\u2010high\u2010performance liquid chromatography\u2010Quadrupole\u2010Exactive\u2010mass spectrometry (UHPLC\u2010QE\u2010MS). Network pharmacology integrated the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database\u2010predicted ACI targets and multiple databases to construct herb\u2010component\u2010target networks (Cytoscape). Core targets were prioritized through protein\u2010protein interaction network analysis (STRING) and topological filtering (degree\/closeness\/betweenness centrality). Pathway enrichment (Kyoto Encyclopedia of Genes and Genomes [KEGG] Database for Annotation, Visualization, and Integrated Discovery) and molecular docking (AutoDock Vina) were performed. Key findings were validated in vivo using a middle cerebral artery occlusion rat model. UHPLC\u2010QE\u2010MS identified 1041 components; 229 were ACI\u2010relevant. Topological analysis revealed 29 core targets (e.g., TP53, STAT3, interleukin [IL]6, tumor necrosis factor [TNF], and Akt1). KEGG enrichment implicated the PI3K\/Akt pathway. Molecular docking confirmed high\u2010affinity binding between HYJDF components (e.g., arachidonic acid and baicalein) and targets (Akt1 and IL6). In vivo, HYJDF treatment upregulated PI3K\/Akt phosphorylation, suppressed neuroinflammation, and attenuated cerebral ischemic injury. HYJDF mediates therapeutic effects against ACI primarily by activating the PI3K\/Akt signaling pathway and downregulating associated inflammatory factors.<\/jats:p>","DOI":"10.1002\/cbdv.202501114","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T20:16:17Z","timestamp":1755548177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deciphering the Pharmacological Mechanism of Huayu Jiedu Formula in Acute Cerebral Infarction by Ultra\u2010High\u2010Performance Liquid Chromatography\u2010Quadrupole\u2010Exactive\u2010Mass Spectrometry, Network Pharmacology, and Experimental Validation"],"prefix":"10.1002","volume":"22","author":[{"given":"Wuchaonan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Integrated Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"},{"name":"Hunan Province Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dingxiang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Integrated Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"},{"name":"Hunan Province Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Integrated Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"},{"name":"Hunan Province Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2756-3831","authenticated-orcid":false,"given":"Yihui","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Hunan University of Chinese Medicine  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00252-0"},{"key":"e_1_2_11_3_1","first-page":"649","article-title":"Changing trends in burden of stroke among residents of China, the United States, and the world during 2010\u20132019: an age\u2010period\u2010cohort analysis of data from GBD 2019","volume":"40","author":"Tang S.","year":"2024","journal-title":"Journal Of Public Health"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/svn-2023-002820"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chmed.2023.09.003"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e36748"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2022.0311"},{"key":"e_1_2_11_8_1","first-page":"843","article-title":"Study on effect of Huayu Jiedu Formula on levels of plasma TAT\uff0cF1 + 2\uff0cD\u2010 dimer and vWF in MCAO rats","volume":"34","author":"Li D. 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