{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:45Z","timestamp":1785330345187,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    is a common opportunistic pathogen in clinical settings, often exhibiting resistance to antifungal therapies. Exploring novel compounds that inhibit\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    holds significant clinical and translational value. Our team recently identified a biphenyl compound 3,4,5\u2010trihydroxy\u2010biphenyl (THB) from\n                    <jats:italic>Sorbus pohuashanensis<\/jats:italic>\n                    ; however, its antifungal activity against\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    and the associated molecular response remain unclear. This study represents the first analysis of THB against\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    based on the minimum inhibitory concentration (MIC) and offers preliminary insights into the molecular mechanism of action through transcriptomic sequencing. The results demonstrated that the MIC of THB against\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    was 1.77\u00a0\u00b5g\/mL. Transcriptomic sequencing revealed 785 differentially expressed genes (DEGs) following treatment of\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    with THB, with 332 genes significantly upregulated and 453 genes significantly downregulated. Gene ontology (GO) analysis indicated that the DEGs were primarily associated with cellular processes, metabolic pathways, and cellular component (CC) organization or biogenesis. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DEGs were primarily enriched in pathways related to translation, carbon metabolism, amino acid metabolism, and energy metabolism. qRT\u2010PCR results confirmed similar expression patterns for\n                    <jats:italic>mak16<\/jats:italic>\n                    ,\n                    <jats:italic>brx1<\/jats:italic>\n                    ,\n                    <jats:italic>CAGL0C03223g<\/jats:italic>\n                    , and\n                    <jats:italic>CAGL0E05984g<\/jats:italic>\n                    as observed in the RNA\u2010Seq analysis. These findings indicate that THB has potent in vitro antifungal activity against\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    , supporting its potential as a lead compound for further antifungal investigation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501137","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:24:33Z","timestamp":1783369473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sterilization Activity and Mechanism of 3,4,5\u2010Trihydroxy\u2010biphenyl From\n                    <i>Sorbus pohuashanensis<\/i>\n                    Against\n                    <i>Candida glabrata<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Yufeng","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Jining high\u2010Throughput Gene Sequencing Key Laboratory of Jining Prenatal Monitoring and Genetic Disease Research Key Laboratory of Jining Pathogen Nanobiotic Prevention and Control New Technology Research, Medical Laboratory Jining NO.1 People's Hospital  Jining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao\u2010di Herbs National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"},{"name":"Research Center for Quality Evaluation of Dao\u2010di Herbs  Ganjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao\u2010di Herbs National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"},{"name":"Research Center for Quality Evaluation of Dao\u2010di Herbs  Ganjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0046-7260","authenticated-orcid":false,"given":"Biao","family":"Chen","sequence":"additional","affiliation":[{"name":"Jining Key Laboratory for the Intelligent Diagnosis of Emerging Infectious Diseases Jining NO.1 People's Hospital Central Laboratory Jining NO.1 People's Hospital  Jining China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11060718"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aambs.2014.12.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2019.02.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770\u2010023\u201000943\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.10.012"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph12040182"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01285"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.820312"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S145545"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2021.104914"},{"key":"e_1_2_10_12_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts","author":"CLSI","year":"2017"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.105867"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.11.027"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.26"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.028"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1792773"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.8.68"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153374"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2016.369"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2022.103955"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202100079"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foaf025"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.07.040"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.72593"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyaf001"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2513844122"}],"container-title":["Chemistry &amp; 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