{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T23:44:36Z","timestamp":1779925476235,"version":"3.53.1"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Otomycosis is a fungal infection of the external auditory canal, predominantly caused by\n                    <jats:italic>Candida<\/jats:italic>\n                    spp. The increasing resistance of these pathogens to conventional antifungal agents requires the exploration of alternative therapeutic approaches. This study was designed to assess the antifungal, anti\u2010virulence, synergistic potential, and mechanism of action of\n                    <jats:italic>Thymus vulgaris<\/jats:italic>\n                    essential oil (EO) against\n                    <jats:italic>Candida<\/jats:italic>\n                    spp., causative agents of otomycosis. Gas chromatography\u2013mass spectrometry (GC\u2013MS) and GC\u2013flame ionization detection (GC\u2013FID) analyses identified\n                    <jats:italic>p<\/jats:italic>\n                    \u2010cymene (38.7%), thymol (30.3%), \u03b3\u2010terpinene (2.6%), carvacrol (3.8%), and linalool (5.2%) as major components. The antifungal activity was assessed using the microdilution method, revealing minimal inhibitory concentrations (MICs) ranging from 0.156 to 2.5\u00a0mg\/mL. Although none of the strains exhibited phospholipase activity, the production of all formed biofilms was significantly inhibited by\n                    <jats:italic>T. vulgaris<\/jats:italic>\n                    EO (21.4%\u201392.8%). The checkerboard assay revealed additive or indifferent interactions with nystatin in most tested strains, suggesting that combined use may offer limited therapeutic benefit. Cholesterol and sorbitol assays showed the affinity of the EO for binding to cholesterol rather than targeting the fungal cell wall. These findings highlight the potential of thyme EO as an alternative antifungal agent for otomycosis treatment, though further research is needed to optimize its therapeutic application.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501145","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T20:55:02Z","timestamp":1757105702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antifungal Activity of\n                    <i>Thymus vulgaris<\/i>\n                    L. Essential Oil Against\n                    <i>Candida<\/i>\n                    Species Causing Otomycosis: Anti\u2010Virulence Activity, Synergistic Potential, and Cell Wall Effect"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1694-3823","authenticated-orcid":false,"given":"Zorica","family":"Stojanovi\u0107\u2010Radi\u0107","sequence":"first","affiliation":[{"name":"Department of Biology and Ecology Faculty of Sciences and Mathematics University of Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3409-4791","authenticated-orcid":false,"given":"Ognjen","family":"Stojkovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology Faculty of Sciences and Mathematics University of Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1342-7567","authenticated-orcid":false,"given":"Niko","family":"Radulovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences and Mathematics University of Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Dimitrijevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences and Mathematics University of Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ran\u0111elovi\u0107","sequence":"additional","affiliation":[{"name":"Medical Faculty University of Ni\u0161  Ni\u0161 Serbia"},{"name":"Public Health Institute Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana","family":"Ota\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Medical Faculty University of Ni\u0161  Ni\u0161 Serbia"},{"name":"Public Health Institute Ni\u0161  Ni\u0161 Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2009.12.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/014556130007900815"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/JOF8030315"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000948"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2020.101011"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8060857"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/JOF9060662"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00405\u2010013\u20102486\u20100\/METRICS"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PMED.0050219"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908\u2010019\u20100702\u20109"},{"key":"e_1_2_9_12_1","volume-title":"Antibiotic Resistance: Mechanisms and New Antimicrobial Approaches","author":"Ch\u00e1vez\u2010Gonz\u00e1lez M. 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