{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:19:52Z","timestamp":1773494392779,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of the present study was to screen for the key hepatoprotective constituents of\n                    <jats:italic>Odontites vulgaris<\/jats:italic>\n                    Moench. As a result, the D30 elution fraction, separated by D101 macroporous adsorption resin column chromatography, had the most prominent protective effect on carbon tetrachloride\u2010damaged HepG2 cells, the most significant 2,2\u2032\u2010azinobis\u2010(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) radical scavenging ability, and the highest total phenol and total flavonoid contents, indicating that the D30 elution fraction may be the active fraction for liver protection. Ultra\u2010performance liquid chromatography analyses revealed that ipolamiide, 8\u2010epiloganin, and acteoside were the main components. Results of the bioactivity evaluation demonstrated that acteoside had hepatoprotective activity, with a half\u2010maximal effective concentration value of 35.32 \u00b1 5.33\u00a0\u00b5M. Results of network pharmacology and molecular docking showed that acteoside had an affinity binding energy of \u22126.53 and \u22125.59\u00a0kcal\/mol to matrix metalloproteinase\u201012 (MMP\u201012) and HSP90AA1, respectively, indicating good binding between acteoside and MMP\u201012 and HSP90AA1. All the findings in the present study reveal that acteoside may be the key hepatoprotective constituent of\n                    <jats:italic>O vulgaris<\/jats:italic>\n                    , which deserves further study.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501171","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T17:20:46Z","timestamp":1758734446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening for the Key Hepatoprotective Constituent From\n                    <i>Odontites vulgaris<\/i>\n                    Moench"],"prefix":"10.1002","volume":"22","author":[{"given":"Yu\u2010ting","family":"Hou","sequence":"first","affiliation":[{"name":"College of Medicine, Institute of Translational Medicine Yangzhou University  Yangzhou China"},{"name":"The Key Laboratory of the Jiangsu Higher Education Institutions For Integrated Traditional Chinese and Western Medicine in Senile Diseases Control Yangzhou University  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Jun","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Neurology Haian Hospital of Traditional Chinese Medicine  Haian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medicine, Institute of Translational Medicine Yangzhou University  Yangzhou China"},{"name":"The Key Laboratory of the Jiangsu Higher Education Institutions For Integrated Traditional Chinese and Western Medicine in Senile Diseases Control Yangzhou University  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Medicine, Institute of Translational Medicine Yangzhou University  Yangzhou China"},{"name":"The Key Laboratory of 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