{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:32:45Z","timestamp":1791873165749,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Allamanda cathartica<\/jats:italic>\n is a perennial shrub, traditionally used for the treatment of several ailments, including jaundice, malaria, and enlargement of the spleen. Besides ornamental values, the plant also possesses a wide variety of medicinal properties due to the presence of bioactive secondary metabolites of different chemical classes. The present work describes the phytochemical investigation of the leaf part of\n A. cathartica<\/jats:italic>\n , which resulted in the isolation of five secondary metabolites (\n ACL 1<\/jats:bold>\n \u2013\n 5<\/jats:bold>\n ) of different classes, including \u03b2\u2010sitosterol (\n ACL\u20101<\/jats:bold>\n ), \u03b2\u2010sitosterol glucoside (\n ACL\u20102<\/jats:bold>\n ), ursolic acid (\n ACL\u20103<\/jats:bold>\n ), plumieride (\n ACL\u20104<\/jats:bold>\n ), and pinitol (\n ACL\u20105<\/jats:bold>\n ). The compound\n ACL\u20105<\/jats:bold>\n was reported for the first time from the species. All the compounds were characterized by\n 1<\/jats:sup>\n H NMR,\n 13<\/jats:sup>\n C NMR, 2D NMR, and HRMS spectral analysis. Further to the plant extract, all the fractions and five isolated compounds were screened for in vitro \u03b1\u2010amylase and \u03b1\u2010glucosidase activity. The isolated compounds exhibited notable inhibitory activity against carbohydrate\u2010hydrolyzing enzymes. For \u03b1\u2010amylase inhibition, IC\n 50<\/jats:sub>\n values ranged from 522.95 to 662.67\u00a0\u00b5g\/mL, with plumieride showing the highest potency at a concentration of 522.95\u00a0\u00b5g\/mL and pinitol acetate the lowest at 662.67\u00a0\u00b5g\/mL. Similarly, for \u03b1\u2010glucosidase inhibition, IC\n 50<\/jats:sub>\n values ranged from 576.82 to 691.12\u00a0\u00b5g\/mL, where plumieride again demonstrated the strongest activity at 576.82\u00a0\u00b5g\/mL and pinitol acetate the lowest at 662.67\u00a0\u00b5g\/mL. In support, all isolated compounds were taken further for molecular modelling studies to observe the rationale of our in vitro studies. Therefore, this work concludes that the isolated compounds\n ACL\u20104<\/jats:bold>\n and\n ACL\u20105<\/jats:bold>\n have demonstrated strong antidiabetic potential, with\n ACL\u20104<\/jats:bold>\n having a somewhat more powerful antidiabetic effect than\n ACL\u20105<\/jats:bold>\n , according to both in vitro and docking experiments.\n <\/jats:p>","DOI":"10.1002\/cbdv.202501183","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T18:00:09Z","timestamp":1759168809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural Characterization, In Vitro Antidiabetic Activity, and Molecular Docking Studies of Isolated Compounds From\n Allamanda cathartica<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Ritu","family":"Tomar","sequence":"first","affiliation":[{"name":"School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy DIT University Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashank Shekher","family":"Mishra","sequence":"additional","affiliation":[{"name":"School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy DIT University Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Sahu","sequence":"additional","affiliation":[{"name":"School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy DIT University Dehradun India"},{"name":"Haldia Institute of Pharmacy Haldia India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagannath","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management NMIMS University Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandeep K.","family":"Arora","sequence":"additional","affiliation":[{"name":"School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy DIT University Dehradun India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8557-9450","authenticated-orcid":false,"given":"Santosh Kumar","family":"Rath","sequence":"additional","affiliation":[{"name":"School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy DIT University Dehradun 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