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Among the solvents examined, ethanol and methanol extracts possess major phytoconstituents, with methanol revealing the higher extractive value (10.45%). Several bioactive substances were found through high\u2010resolution liquid chromatography mass spectrometry (HR\u2010LCMS) analysis. The ethanolic extract had higher total phenolic (218.6\u00a0\u00b1\u00a04.16\u00a0mg gallic acid equivalent [GAE]\/g) and flavonoid (256.5\u00a0\u00b1\u00a03.13\u00a0mg quercetin equivalent [QE]\/g) contents, whereas the methanolic extract had higher terpenoid content (144.64\u00a0\u00b1\u00a02.03\u00a0mg\u00a0LNE\/g). The ethanolic extract had high antioxidant activity, including DPPH scavenging (58.47%\u00a0\u00b1\u00a00.18%), phosphomolybdate (60.54%\u00a0\u00b1\u00a00.15%), and metal chelation (64.91%\u00a0\u00b1\u00a00.54%). The BSA assay demonstrated anti\u2010inflammatory activity (59.07%\u00a0\u00b1\u00a00.02%). At 100\u00a0\u00b5g\/mL, the ethanolic extract demonstrated antibacterial action against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Bioassays using brine shrimp and hemolysis showed moderate toxicity. HeLa (54.38%\u00a0\u00b1\u00a00.81%) and MCF\u20107 (51.37%\u00a0\u00b1\u00a01.22%) cells showed reduced viability in the cytotoxicity assay by ethanolic extract. In silico docking confirmed strong binding affinities of the identified anticancer compounds to target proteins. This work presents novel HR\u2010LCMS profiling and anticancer evaluation of\n                    <jats:italic>C. apetalum<\/jats:italic>\n                    leaf metabolites.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501331","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T13:58:57Z","timestamp":1761832737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HR\u2010LCMS\u2010Guided Phytochemical Profiling, Multi\u2010Target Bioactivities, and In Silico Studies of\n                    <i>Calophyllum apetalum<\/i>\n                    Willd. 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