{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:47:54Z","timestamp":1786628874420,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A series of 2\u2010(2\u2010arylidenehydrazinyl)\u20104,6\u2010bis(substituted\u2010phenoxy)\u20101,3,5\u2010triazines (compounds\n                    <jats:bold>3a<\/jats:bold>\n                    \u2013\n                    <jats:bold>o<\/jats:bold>\n                    to\n                    <jats:bold>7a<\/jats:bold>\n                    \u2013\n                    <jats:bold>o<\/jats:bold>\n                    ) was synthesized and evaluated for DNA\u2010binding affinity using calf thymus DNA. Based on their DNA interaction profiles, the top three compounds from each series were selected for in vitro anticancer screening against eight human cancer cell lines, encompassing both solid tumors (glioblastoma, pancreatic adenocarcinoma, colorectal carcinoma, and lung carcinoma) and hematological malignancies (acute lymphoblastic leukemia, acute myeloid leukemia, chronic myeloid leukemia, and non\u2010Hodgkin lymphoma). Among the tested compounds,\n                    <jats:bold>5i<\/jats:bold>\n                    and\n                    <jats:bold>7b<\/jats:bold>\n                    demonstrated the highest anticancer potency against pancreatic adenocarcinoma (Capan\u20101), with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 2.4 and 1.9\u00a0\u00b5M, respectively. Notably, compound\n                    <jats:bold>5i<\/jats:bold>\n                    also exhibited strong cytotoxicity against colorectal carcinoma (HCT\u2010116), with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 2.2\u00a0\u00b5M. Additional analogues namely\n                    <jats:bold>3i<\/jats:bold>\n                    ,\n                    <jats:bold>3l<\/jats:bold>\n                    ,\n                    <jats:bold>6g<\/jats:bold>\n                    ,\n                    <jats:bold>6l<\/jats:bold>\n                    , and\n                    <jats:bold>7m<\/jats:bold>\n                    displayed IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 7.4 to 42.2\u00a0\u00b5M across the panel of cancer cell lines, highlighting the superior activity of\n                    <jats:bold>5i<\/jats:bold>\n                    and\n                    <jats:bold>7b<\/jats:bold>\n                    as promising antiproliferative agents. Molecular docking studies revealed that compound\n                    <jats:bold>7b<\/jats:bold>\n                    formed strong interactions with the prenyl\u2010binding protein PDE\u03b4, and subsequent molecular dynamics simulations confirmed the stability of the\n                    <jats:bold>7b<\/jats:bold>\n                    \u2013PDE\u03b4 complex, supporting its potential as a targeted anticancer agent.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501362","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T18:10:09Z","timestamp":1753899009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Synthesis of Novel 1,3,5\u2010Triazine Analogues With Selective Activity Against Pancreatic (Capan\u20101) and Colorectal (HCT\u2010116) Carcinomas: Anticancer Potential and DNA\u2010Binding Affinity"],"prefix":"10.1002","volume":"22","author":[{"given":"Shazia","family":"Asghar","sequence":"first","affiliation":[{"name":"Department of Chemistry Quaid\u2010I\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aftab","family":"Aziz","sequence":"additional","affiliation":[{"name":"Department of Chemistry Quaid\u2010I\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1195-1659","authenticated-orcid":false,"given":"Shahid","family":"Hameed","sequence":"additional","affiliation":[{"name":"Department of Chemistry Quaid\u2010I\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahjat A.","family":"Saeed","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Education for Pure Science University of Basrah  Basrah Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risala H.","family":"Allami","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical biotechnology, College of Biotechnology Al\u2010Nahrain University  Baghdad Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6811-191X","authenticated-orcid":false,"given":"Najim A.","family":"Al\u2010Masoudi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science University of Basrah  Basrah 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