{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:49:16Z","timestamp":1788929356525,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the biocontrol potential of culture filtrates and secondary metabolites (SMs) from the nematophagous fungus\n                    <jats:italic>Pochonia chlamydosporia<\/jats:italic>\n                    . The fungus exhibited antifungal activity against pathogens such as\n                    <jats:italic>Pythium myriotylum<\/jats:italic>\n                    ,\n                    <jats:italic>Macrophomina<\/jats:italic>\n                    sp.,\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    sp.,\n                    <jats:italic>Phytophthora<\/jats:italic>\n                    sp.,\n                    <jats:italic>Fusarium<\/jats:italic>\n                    sp., and\n                    <jats:italic>Exerohilum rostratum<\/jats:italic>\n                    . The highest percentage inhibition of radial growth values was observed with\n                    <jats:italic>P. myriotylum<\/jats:italic>\n                    (81.42%) and\n                    <jats:italic>Phytophthora<\/jats:italic>\n                    sp. (82.71%), while\n                    <jats:italic>Macrophomina<\/jats:italic>\n                    sp. showed the lowest inhibition (40%). The study also aimed to identify the SMs responsible for the biocontrol activity of\n                    <jats:italic>P. chlamydosporia<\/jats:italic>\n                    . Secondary metabolites were extracted using ethyl acetate, and their activity profiles were assessed via thin\u2010layer chromatography. The ethyl acetate extracts fully inhibited the mycelial growth of\n                    <jats:italic>P. myriotylum<\/jats:italic>\n                    (99.54%) and\n                    <jats:italic>Phytophthora<\/jats:italic>\n                    sp. (99.99%). Moreover, the extract demonstrated significant nematicidal activity against the plant\u2010parasitic nematode (PPN)\n                    <jats:italic>Radopholus similis<\/jats:italic>\n                    , with the crude extract showing the highest efficacy among various concentrations tested. High\u2010resolution ultra\u2010performance liquid chromatography\u2010electrospray ionization\u2010quadrupole time\u2010of\u2010flight mass spectrometry analysis of the extract revealed a diverse array of bioactive compounds, including antifungal, antibacterial, antiviral, antitumor, cytotoxic, antimalarial, antitrypanosomal, and nematicidal agents. An in\u2010silico screening approach was employed to identify potential lead compounds for nematicidal activity, targeting glutathione S\u2010transferases and venom allergen\u2010like proteins. The results highlighted three compounds, bisevertinol, enniatin I, and enniatin H, that exhibited strong binding affinities to multiple pharmacological targets, suggesting their potential as lead candidates for developing nematicidal agents against PPNs.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501418","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T19:51:56Z","timestamp":1755114716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the Biocontrol Potential of\n                    <i>Pochonia chlamydosporia<\/i>\n                    : Antifungal and Nematicidal Activities of Culture Filtrates and Secondary Metabolites"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-858X","authenticated-orcid":false,"given":"Mery Rincy","family":"K","sequence":"first","affiliation":[{"name":"Division of Crop Protection ICAR\u2010Indian Institute of Spices Research  Kozhikode India"},{"name":"University of Calicut  Malappuram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santhosh J.","family":"Eapen","sequence":"additional","affiliation":[{"name":"Division of Crop Protection ICAR\u2010Indian Institute of Spices Research  Kozhikode India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveena","family":"R","sequence":"additional","affiliation":[{"name":"Division of Crop Protection ICAR\u2010Indian Institute of Spices Research  Kozhikode India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1149947"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-017-8730-7_7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2916"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.02.016"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2014.02.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2010.01.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42483-024-00257-6"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/np020556v"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2009.02.027"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8010062"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/np0301285"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1np00039j"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3NP70097F"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbas.2018.10.001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-014-0454-x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13010024"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.9b04332"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol052263"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr700742h"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-4998(200012)56:12<1098::AID-PS244>3.0.CO;2-H"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b02595"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12057"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2010.00614.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-22-2-0115"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2011.10.040"},{"issue":"1","key":"e_1_2_6_27_1","first-page":"173","article-title":"Cloning and Characterization of Genes Encoding Two Detoxifying Enzymes, Glutathiones Transferase and Carboxylesterase, From Burrowing Nematode (Radopholus similis)","volume":"8","author":"Rosana O. 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