{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:06:39Z","timestamp":1787065599079,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In the pursuit of novel antioxidant and anti\u2010inflammatory agents, we investigated 5\u2010hydroxyferulic acid (5\u2010OHFA), a hydroxylated derivative of the well\u2010known phenolic compound ferulic acid (FA). This study aimed to determine whether structural modification enhances the biological activity of FA. To this end, both compounds were subjected to a series of in vitro antioxidant assays (DPPH, ABTS, FRAP, and Fe(II)\u2010chelating) and anti\u2010inflammatory evaluations, complemented by in silico predictions. 5\u2010OHFA consistently exhibited superior antioxidant capacity, with significantly lower IC\n                    <jats:sub>50<\/jats:sub>\n                    values than FA based on literature\u2010reported values: 11.89\u00a0\u00b1\u00a00.20 versus 66\u00a0\u00b1\u00a02.3\u00a0\u00b5M (DPPH), 9.51\u00a0\u00b1\u00a00.15 versus 183.08\u00a0\u00b1\u00a02.30\u00a0\u00b5M (ABTS), 5.94\u00a0\u00b1\u00a00.09 versus 4.73\u00a0\u00b1\u00a00.14\u00a0\u00b5M (FRAP), and 36.31\u00a0\u00b1\u00a01.36 versus 270.27\u00a0\u00b1\u00a01.14\u00a0\u00b5M (Fe\n                    <jats:sup>2+<\/jats:sup>\n                    chelation). It also demonstrated stronger anti\u2010inflammatory potential in protein denaturation assays using egg albumin and bovine serum albumin (BSA). Although 5\u2010OHFA showed slightly greater hemolytic activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a023.78\u00a0\u00b1\u00a01.48\u00a0\u00b5M) than FA, based on literature\u2010reported values (37.64\u00a0\u00b1\u00a02.01\u00a0\u00b5M), both remained within biologically acceptable limits. In silico analyses using SwissADME and ProTox III supported the experimental findings, predicting good oral bioavailability, high gastrointestinal absorption, mild blood\u2013brain barrier permeability, and no significant toxicity for 5\u2010OHFA. Molecular docking studies further revealed stronger binding affinities of 5\u2010OHFA to key oxidative and inflammatory targets, including NADPH oxidase (2CDU), xanthine oxidase (1FIQ), 5\u2010lipoxygenase (3O8Y), cyclooxygenase\u20102 (3LN1), myeloperoxidase (1DNU), and the EGFR enzyme's active pocket (1M17). Overall, the data suggest that 5\u2010OHFA possesses enhanced bioactivity relative to its parent compound FA, supporting its potential as a promising multifunctional candidate for pharmaceutical or nutraceutical development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501431","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:34:41Z","timestamp":1758821681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrated In Vitro and In Silico Characterization of 5\u2010Hydroxyferulic Acid: Antioxidant, Anti\u2010Inflammatory, Anti\u2010Hemolytic, and Cytotoxic Potential"],"prefix":"10.1002","volume":"22","author":[{"given":"Syrine","family":"Sakouhi","sequence":"first","affiliation":[{"name":"Laboratory of Population Health, Environmental Aggressors and Alternative Therapies (LR24ES10), Faculty of Medicine of Tunis University of Tunis\u2010El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3765-991X","authenticated-orcid":false,"given":"Sonia","family":"Ben Younes","sequence":"additional","affiliation":[{"name":"Laboratory of Population Health, Environmental Aggressors and Alternative Therapies (LR24ES10), Faculty of Medicine of Tunis University of Tunis\u2010El Manar  Tunis Tunisia"},{"name":"Faculty of Medicine of Sousse University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Arrari","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources, Higher Institute of Biotechnology of Beja University of Jendouba  Beja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afef","family":"Nahdi","sequence":"additional","affiliation":[{"name":"Laboratory of Population Health, Environmental Aggressors and Alternative Therapies (LR24ES10), Faculty of Medicine of Tunis University of Tunis\u2010El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalloul","family":"Bouajila","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique Universit\u00e9 de Toulouse, CNRS, INP, UT  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abada","family":"Mhamdi","sequence":"additional","affiliation":[{"name":"Laboratory of Population Health, Environmental Aggressors and Alternative Therapies (LR24ES10), Faculty of Medicine of Tunis University of Tunis\u2010El Manar  Tunis 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